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Master Thesis

Transplantation pulmonaire et dysfonction chronique du greffon‎ : caractérisation de biomarqueurs diagnostiques par analyse protéomique SELDI-TOF du liquide bronchiolo-alvéolaire

Abstract : Objective: Despite progresses in lung transplantation, long-term prognosis is compromised by chronic allograft rejection. Its diagnosis is based on a delayed functional criterion and its physiopathology is still unclear. We investigated proteomic profiles of bronchoalveolar lavage fluid (BALF) of lung transplanted patients by an innovative proteomic technology in order to characterize diagnostic biomarkers of chronic allograft rejection. Methods: In a cross sectional pilot study, protein profiles of 13 lung transplant recipients with bronchiolitis obliterans syndrome (BOS), 21 patients with lung function stability (ST) and 8 patients with acute rejection (AR) were studied by SELDI-TOF MS (Surface Enhanced Laser Desorption Ionization Time-Of-Flight Mass Spectrometry) analysis. Results: Forty five differentially expressed proteins were detected in BOS group vs. ST population and forty two in BOS group vs. AR set. Among the most discriminating peaks, biomarkers corresponding to S100 proteins (10,812 Da, 10,412 Da and 13,335 Da, p<0.002) and alpha-defensins (3,354 Da, 3,424 Da and 3,470 Da, p<0.01) were up regulated in BOS patients compared to ST or AR patients. S100A8 protein (10,812 Da) discriminate BOS patients from ST patients with a sensitivity of 92% and a specificity of 72% (AUC=0.87), while alpha-defensins offer a slightly weaker diagnostic accuracy. Conclusion: BALF SELDI-TOF analysis allows detection of relevant biomarkers linked to BOS diagnosis and potentially involved in its physiopathology: S100 proteins and alpha-defensins.
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Master Thesis
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https://dumas.ccsd.cnrs.fr/dumas-00631137
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Submitted on : Tuesday, October 11, 2011 - 3:21:10 PM
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  • HAL Id : dumas-00631137, version 1

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Sébastien Quetant. Transplantation pulmonaire et dysfonction chronique du greffon‎ : caractérisation de biomarqueurs diagnostiques par analyse protéomique SELDI-TOF du liquide bronchiolo-alvéolaire. Médecine humaine et pathologie. 2009. ⟨dumas-00631137⟩

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