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Master Thesis

Rôle des voies de signalisation de deux protéines kinases, Erk et Akt, dans l'effet anti-œdémateux de l'érythropoïétine

Abstract : Our team found that erythropoietin (EPO) significantly reduced the development of brain edema in a rat model of diffuse traumatic brain injury (TBI). This reduction was associated with early changes of brain phosphorylation of two protein kinases, Erk and Akt. In this study, we investigated the possible causal link between phosphorylation changes and brain edema. To specifically inhibit Erk and Akt, we developed a model with intracerebroventricular injection by using intracerebral injection of Indian ink and optical microscopy visualization. The ERK-1/-2 inhibitor, U0126, or the Akt inhibitor, LY294002 were administered before traumatic brain injury. EPO (5,000 IU/kg body weight) was intravenously administrated 30 mins after injury brain. Phosphorylation of Erk and Akt was measured 1 hour after insult (H1) and brain water content were measured 2 hours (H2) after insult by gravimetric technique. The inhibition of Akt pathway did not result in significant differences in brain water contain (BWC); the addition of post injury treatment with EPO significantly decreased BWC. Inhibition of Erk reduced BWC, no further reduction of brain edema was found when Erk was combined with EPO treatment. These results suggest that the anti-edematous effect of EPO after diffuse TBI could be mediated through an early inhibition of ERK phosphorylation.
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Master Thesis
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https://dumas.ccsd.cnrs.fr/dumas-00631166
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Submitted on : Thursday, May 17, 2012 - 3:38:03 PM
Last modification on : Tuesday, May 11, 2021 - 11:36:16 AM
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Marie-Cécile Fèvre. Rôle des voies de signalisation de deux protéines kinases, Erk et Akt, dans l'effet anti-œdémateux de l'érythropoïétine. Médecine humaine et pathologie. 2011. ⟨dumas-00631166⟩

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