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Le prélèvement doit se réaliser sous asepsie stricte Le scalp est visualisé par l'amnioscope, le pôle céphalique est nettoyé (présence de LA, sang maternel, graisse foetale) avec de l'éther puis enduit de vaseline, dans le but de faire perler le sang. Une incision est pratiquée (profondeur maximum de 2 mm), la première goutte de sang est éliminée ,