Skip to Main content Skip to Navigation
Master Thesis

Développement d’approches innovantes pour la production d’anticorps monoclonaux humains : application au modèle de la dengue

Abstract : This work performed in the context of the Hu's map project, allowed the development of innovative approaches to generate human B-cell derived monoclonal antibodies. We notably attempt to optimize B cell survival by the use of fusogenic vesicles able to deliver factors promoting immortalization. Among these, the transcriptionnal activator EBNA2 protein of EBV was particularly studied. By transferring EBNA2 into recipient B-cells infected by EBV, we were able to potentalize immortalization and significantly increase IgG production. This method of immortalization assisted by EBNA2-vesicles wasnext applied to the production of human antibodies against the dengue virus, which remains a major public health concern. Several B-cell clones producing human monoclonal antibodies recognizing dengue's epitope have been obtained and are under characterization.
Document type :
Master Thesis
Complete list of metadatas

Cited literature [101 references]  Display  Hide  Download

https://dumas.ccsd.cnrs.fr/dumas-01081521
Contributor : Cnam - Service Commun de la Documentation - Bibliothèque Centrale <>
Submitted on : Saturday, November 8, 2014 - 4:56:04 PM
Last modification on : Saturday, February 8, 2020 - 12:10:49 PM
Long-term archiving on: : Monday, February 9, 2015 - 10:31:28 AM

Licence


Distributed under a Creative Commons Attribution - NonCommercial - NoDerivatives 4.0 International License

Identifiers

  • HAL Id : dumas-01081521, version 1

Collections

Citation

Caroline Majenski. Développement d’approches innovantes pour la production d’anticorps monoclonaux humains : application au modèle de la dengue. Biotechnologies. 2011. ⟨dumas-01081521⟩

Share

Metrics

Record views

309

Files downloads

548