L. Cellules, M. , and M. , 44 I ).46 I.1 )Echantillons utilisés, .47 I.1.1.2 )PCR Allèle, p.49

I. Contrôle-du-phénotype-lu and .. , 50 II.1.1 )Isolements des glomérules51 II.1.2 )Coloration Immuno-Histo-Fluorescence des glomérules52 II.1.4 )Dosage des protéines glomérulaires52 II.1.5 )Co-précipitation des protéines, 50 II.1 )57 III ) PREPARATION D'UN NOUVEL «APPÂT» Lu/BCAM PAR CLONAGE.........................57 III.1 )Préparation de la matrice, p.60

.. Da-)-pour-la-ligation and D. Et, 61 III.2.1 )Préparation de l'insert61 III.2.2 )Préparation des vecteurs N3C, III.2 )Préparation de l'insert des plasmides contenant la partie extracellulaire non mutée (N3C) et mutante, p.65

C. Du and C. , 66 IV.1 )Extraction de plasmides66 IV.2 )Contrôle de la mini-préparation d, ADN : vecteur + insert, p.68

M. , V. Lu, and /. , 73 VII.1 )Western Blot, 70 VII ), p.74

R. Au-nitrate-d-'argent, . Des, A. Co-precipitees-avec-l-'appât-fc-lu, . Partir, . Extraits et al., 76 I )76 II )77 III ), 80 III.2 )REVELATION82 III.3 )REVELATION, p.83

V. Par, . Blot, .. Co-p-fc-lu, and L. De-l-'anticorps, 87 VI.1 )ANALYSE, p.90

V. Nouvel-appât-luic, .. De-l-'insert, and .. , 91 VII.1 ), p.92

C. Dernier-contrôle-sur-notre-Échantillon-de, /. Fc-lu, and R. Ko-en, à l'aide de l'anticorps anti laminine ?5 ultra-purifié (Ac L85 P52) en conditions non réduites et réduites (Figure 36) On obtient le résultat inverse par rapport aux cellules murines bEnd.3. La présence d'une bande à 400kDa en conditions non réduites, mais absente en conditions réduites. Tous ces résultats confirment que nous n'avons pas co-précipité la laminine

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A. Experimental, Hébergement : 1,03? HT/souris soit 3X donc 3,09? HT/test Perfusion de billes : 47,44? HT/souris soit 3X donc 142,32? HT/test Prélèvement (reins) : 26,15? HT/souris soit 3X donc 78, 45? HT/test Coût global de l'ICS : 223.86? HT/expérience SARL MEG Import/Export

6. Ht-carboglace and H. , Paris : 190? HT Coût du transport : 302.69? HT soit 15,13 à 30,27? HT/souris Isolement des glomérules Solution HBSS 1X : 2,47? HT/test Solution de collagénase 60X : 9,30? HT/test Solution DNase 100X : 6, 2? HT /test Coût de l'expérience : 42,97? HT/expérience Immuno-Histo-Fluorescence Solution PBS 1X : 6,63? HT soit 3X donc 1990? HT/test Anticorps Lu : 0,20? HT/test Anti anticorps Lu : 0,18? HT/test Anticorps ?1 laminine, pp.13-020

H. Coût-de-l-'expérience, 20? HT/expérience Électrophorèse des protéines Gel 3 à 8% Tris Acétate SDS : 18,10? HT/test Marqueur protéine Plus 3, 50? HT/test Tris Acétate, vol.20, issue.3, p.2733

. Coût, expérience : 13,14? HT/expérience La spectrométrie de masse Préparation des échantillons : 25? HT soit 6X donc 150? HT/test Mesures, pp.60-63