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Master Thesis

Étude génétique du stress de contention chez le porc

Abstract : This essay is part of the SUSoSTRESS projet. One of its aims is to study stress responses of farm animals. 120 pigs were subject to a 10 minutes restraint stress. Blood samples were taken to determine cortisol, glucose, lactic acid, free fatty acids, creatine kinase concentrations in plasma. Quantification of plasma catecholamines has been done by high pressure liquid chromatography with electrochemical detection. Behavioral and genetic data were also generated by the project. Data integration aimed to better understand stress responses of our phenotypes and to determine the importance of the autonomic nervous system in those responses. A principal component analysis and Pearson’s correlations allowed us to detect relationships between glucose, lactate and cortisol in a first hand, and between activity, cardiac responses to motions, creatine kinase and cardiac rhythm in a second hand. A genome-wide association analysis was performed to better understand those responses, but the small number of animal in our study did not allow us to reach significant results. An association analysis with candidate genes (dopamine receptor D3, dopamine β-hydroxylase and phenylethanolamine N-methyltransferase genes) was done. Significant associations showed the important role of the autonomic nervous system in controlling stress responses of our phenotypes.
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Master Thesis
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Submitted on : Wednesday, March 25, 2015 - 2:45:12 PM
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Marion Canaple. Étude génétique du stress de contention chez le porc. Sciences agricoles. 2014. ⟨dumas-01135559⟩

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