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Master Thesis

Caractérisation et purification de molécules cages de type cryptophane et cucurbituril pour applications en IRM Xenon

Abstract : Medical imaging (MRI) has become an essential tool in the diagnosis of many diseases. New methods using hyperpolarized noble gases (129Xe, 3He) have been developed and have proven their efficiency in increasing the sensitivity of MRI. Due to its hydrophobic property, the hyperpolarized xenon can be trapped and transported within molecular capsules designed to target biological receptors. Some of those molecular hosts were synthesized in the laboratory and functionalized to obtain water-soluble biosensors. The subject of this report is the optimization of separation methods (LC-UV- MS- ELSD) in order to characterize two types of molecular hosts: the cucurbiturils and cryptophanes. The first part of the document is devoted to the experiments concerning the purification of mixed cucurbiturils CB6Cy[n] with n = 1-6. Furthermore, CB6Cy [n] obtained from various synthetic routes were quantified using a method of internal calibration validated by performance criteria. Reaction yields and water solubility were also determined. The second part of the document is devoted to the study of chiral properties of cryptophanes and the purification of two xenon-biosensors cryptophanes functionalized for medical applications. After purifications, those cages were studied in vitro in the 129Xe NMR experiments confirming the great potential of this approach.
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Master Thesis
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Sylvie Coudert. Caractérisation et purification de molécules cages de type cryptophane et cucurbituril pour applications en IRM Xenon . Sciences de l'ingénieur [physics]. 2011. ⟨dumas-01157683⟩

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