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Master Thesis

Étude expérimentale de l'action de trois édulcorants sur deux bactéries buccales potentiellement cariogènes

Abstract : High-intensity sweeteners, like acesulfame K, sucralose and aspartame, are more and more used as a substitute for sucrose, in food products and in medication. These sweeteners are considered non-cariogenic, due to their different composition of sucrose. However, if much work has already been carried out on polyols (xylitol, sorbitol…), few studies have been published on potentially cariogenic effects of high-intensity sweeteners. The aim of this thesis was to determine if these sweeteners impacted on the caries process. For this, we used two particular bacteria involved in this process : Streptococcus mutans, pioneer bacteria in the formation of caries cavity, at the enamel level, and Lactobacillus rhamnosus, more particularly involved in the progression of the lesion in the dentin. The study focused on the inhibition of growth, of acid production and of extracellular polysaccharides production, allowing adhesion. Analysis of bacterial growth and pH measurements were carried out by culturing bacteria in fermentation minimal medium (FMM), devoid of sugar, which we added different doses of sweeteners. Adhesion was studied according to the method of Hamada and Torii, on glass tubes inclined. Our results show an inhibition of S. mutans’ growth in the presence of acesulfame-K and aspartame. Sucralose does not cause inhibition. Concerning L. rhamnosus, none of the three sweeteners has led to inhibition. The presence of sugar substitutes do not result in medium pH changes. To study the adhesion, no inhibition was observed, regardless of the tested sweetener to S. mutans. Concerning L. rhamnosus alone acesulfame K inhibited the adhesion. Based on our results, the non-cariogenic character of these sweeteners has not been demonstrated. Further studies are needed to test the impact of these synthetic sugars on all oral bacteria to confirm or refute our results.
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Master Thesis
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Submitted on : Monday, October 26, 2015 - 1:50:00 PM
Last modification on : Wednesday, January 15, 2020 - 9:03:57 AM
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Mathieu Sallaberry. Étude expérimentale de l'action de trois édulcorants sur deux bactéries buccales potentiellement cariogènes. Chirurgie. 2015. ⟨dumas-01220450⟩

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