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Master Thesis

Caractérisation des cellules épithéliales mammaires chez la vache laitière

Abstract : Milking production is currently facing new challenges such as the end of the limit of annual milk’s production in the European Union and the realization of the impact on the environment of cow’s breeding. On the other hand, the industry has to produced more to answer the increase of dairy products. Thus the research on dairy cow and especially the bovine mammary gland is important. The mammary gland is a dynamic organ composed by different populations such as mammary stem cells, progenitor and differentiated cells, myoepithelial cells, adipocytes, fibroblasts and endothelial cells. All these population of cells contributed to milking production. Some surface cells markers such as CD49f, CD24, CD10 and EpCAM and the KRT49 gene are used to phenotype these cells. We used these makers to characterize the mammary stem progenitor and differentiated cells and myoepithelial cells during lactation and between two stages of development (heifer / lactating cow). We discovered that most of the populations stained were constant during our experiments. One population with regenerative properties decreased during lactation and the other variations concerned epithelial cell type at the beginning of lactation and were linked to the quantity of milk production. This stability among the cells could be due to the fixation of cell populations during the development of the mammary gland. To test this hypothesis, a work on a 7 month-old heifer must be done. On the other hand, the stem cells’ reservoir could be situated in the adipocytes population. An extensive study of this population of cell should improve our understanding of the evolution of the mammary gland.
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Submitted on : Friday, January 8, 2016 - 10:50:44 AM
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  • HAL Id : dumas-01252765, version 1



Florence Dufreneix. Caractérisation des cellules épithéliales mammaires chez la vache laitière . Sciences du Vivant [q-bio]. 2015. ⟨dumas-01252765⟩



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