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. Rq, Vérifier que tout le surnageant est bien passé au travers de la colonne

. Pour-l-'eeq and . Aglae, prévoir éventuellement une dilution des échantillons dans

I. Kit, Check Legionella pneumophila -Kit IQ Check Legionella spp -Kit d'extraction Aquadien -Echantillons extraits -Pipettes dédiées : 10 µl

. Dans-le-bureau-technicien, Biorad CFX Manager IDE" en double cliquant sur l'icône

. Sélectionner-dans-la-fenêtre-À-gauche, Type" le mode quantitatif dans le menu déroulant puis cliquer sur OK. Cliquer en dessous sur "Legio. pneumo

. Sélectionner, QS1" puis sélectionner à nouveau 2 puits en dessous et cliquer sur

R. Les, B. Rentrer-le-numéro-d-'échantillon, and . Cliquer-sur, Recommencer l'opération autant de fois que nécessaire. Terminer par l'échantillon "matériau de référence, Il est aussi possible de faire le plan de plaque en considérant les puits similaires de façon verticale et non pas horizontale

. Dans-l-'onglet, Table ViewSample type" afin que les répliquats des standards ou échantillons se situent à la suiteStarting Quantity") ainsi que le volume filtré des échantillons s'il est différent de 1 litre (ex : 0.2 pour 200ml)

. Une-fenêtre, Open Plate" s'ouvre, cliquer surBureauLabo d'hygiène" puis "Plan de plaque

. Rq, Tester la connection du PC et du CFX 96 : Fenêtre IDE Plate Manager du logiciel, cliquer sur "Start Run" puis sur "Open Lid". Si le couvercle ne s'ouvre pas

P. Lorsque-la and . Est-terminée, écran suivant apparaît : 1. Droite de calibrage 1.1. Vérification de la pente a : a = -3.38 dans le tableau Excel 1.2. Vérification de l'ordonnée b : b = 39