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Master Thesis

Microchimérisme et prédiction des complications de la grossesse chez les femmes primigestes

Abstract : Objective: To compare fetal microchimerism levels in serum from primigravid women at 12 weeks of amenorrhea (WA), with or without pathological pregnancies. To show that high levels of fetal microchimerism could predict emergence of preeclampsia in women, and/or intra-uterine fetal growth restriction (IUGR) and/or a small of gestational age (SGA) in newborns as early as 12 WA. Methods: Selection of serum from primigravid women with a male or female offspring and with a healthy or pathological pregnancy (with preeclampsia, arterial hypertension, a newborn with IUGR or SGA). Quantification of total circulating DNA by quantitative PCR for β-globin (gene on autosomal chromosome) and for male DNA with SRY PCR (gene on the Y chromosome) on the 4 serum groups. Correlation of microchimerism quantities with 1st trimester serum markers as well as other pregnancy and delivery clinical data. Results: Male fetal microchimerism is more prevalent in women with pathological pregnancies. The analysis shows that high quantities of male microchimerism have a good predictive value for pathological pregnancies (p<0.0001). In the current study this predictive value is better than PAPP-A and β-HCG rates. Male microchimerism can predict a small birth weight. Conclusion: Fetal microchimerism quantification can help predicting preeclampsia and/or IUGR, and/or SGA. Using it as a predictive factor needs detection and quantification protocol standardization. Exploring the creation of a scoring system using microchimerism values in combination with serum markers and patient history to identify preeclampsia high risk women with the objective of preventive therapies would be an interesting follow through.
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https://dumas.ccsd.cnrs.fr/dumas-01417236
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Submitted on : Thursday, December 15, 2016 - 2:30:18 PM
Last modification on : Monday, July 6, 2020 - 4:04:37 PM
Long-term archiving on: : Thursday, March 16, 2017 - 6:00:54 PM

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  • HAL Id : dumas-01417236, version 1

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Érika Vigorito. Microchimérisme et prédiction des complications de la grossesse chez les femmes primigestes. Gynécologie et obstétrique. 2016. ⟨dumas-01417236⟩

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