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Master Thesis

Expression et localisation de la phospholipase C zeta (PLCζ) dans les spermatozoïdes d’hommes fertiles et infertiles

Abstract : Introduction: ovocyte activation is a crucial step to initiate embryo development and achieve pregnancy. This step is triggered by the release of a sperm factor into the ooplasm during gamete fusion : Phospholipase C zeta (PLCζ). Material and methods: in this study, we aimed to evaluate the proportion of sperm exhibiting PLCζ and the localization patterns of this protein in 17 men with proven fertility, 7 patients experiencing unexplained infertility (ID group) and 6 patient who experienced fertilization failure or low fertilization rates in IVF or ICSI (EF group). Results: we didn’t find any significant difference between controls and patients in the proportion of sperm exhibiting PLCζ. In control men, the protein seems to be mainly localized in the equatorial segment, but also in acrosomal and post acrosomal regions. Patients of group 2 exhibited the same pattern of localization. 3 out of 6 patients experiencing fertilization failure in IVF showed low levels of equatorial forms of PLCζ. Conclusion: equatorial localization of PLCζ seems to play a crucial role in oocyte activation process, allowing PLCζ to get easily into the ooplasm. Low levels of equatorial forms could be associated with fertilization failure or low fertilization rates in IVF.
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Master Thesis
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https://dumas.ccsd.cnrs.fr/dumas-01657512
Contributor : Bu Carreire Université de Bordeaux <>
Submitted on : Wednesday, December 6, 2017 - 6:09:51 PM
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Réda Benmansour. Expression et localisation de la phospholipase C zeta (PLCζ) dans les spermatozoïdes d’hommes fertiles et infertiles . Médecine humaine et pathologie. 2017. ⟨dumas-01657512⟩

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