Skip to Main content Skip to Navigation
Master Thesis

Apport de l'immunophénotypage monocytaire sanguin dans le diagnostic de la leucémie myélomonocytaire chronique

Abstract : A new diagnostic marker of the chronic myelo-monocytic leukemia (CMML), based on the phenotype of circulating monocytes by flow cytometry, has been recently published by Selimoglu-Buet et al. Thus, a percentage of monocytes that do not express CD16 (MO1) greater than or equal to 94% of total monocytes has excellent specificity and sensitivity for diagnosis of CMML. In our laboratory, the results of the complete blood count are made by the analyzer couple DXH800 - flow cytometry with the solution Hématoflow (Beckman Coulter, Brea, CA). This strategy provides the percentage of MO1 on a routine blood count. The main objective of this work is to demonstrate the interest of the HematoFlow (HF) for the diagnosis of CMML, through a retrospective study conducted at the University Hospital of Rennes. Secondarily, on a prospective cohort we studied the correlation between HF and conventional flow cytometry for the quantification of MO1, actually implemented in our diagnostic work-up for chronic monocytosis. Finally, we evaluated the relevance of the association of two techniques for the diagnosis of CMML.
Document type :
Master Thesis
Complete list of metadatas

Cited literature [57 references]  Display  Hide  Download

https://dumas.ccsd.cnrs.fr/dumas-01758757
Contributor : Santé Rennes 1 <>
Submitted on : Tuesday, June 5, 2018 - 2:01:20 PM
Last modification on : Thursday, July 9, 2020 - 12:30:44 PM

File

LEMOUELMarc.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : dumas-01758757, version 1

Collections

Citation

Marc Le Mouël. Apport de l'immunophénotypage monocytaire sanguin dans le diagnostic de la leucémie myélomonocytaire chronique. Sciences du Vivant [q-bio]. 2016. ⟨dumas-01758757⟩

Share

Metrics

Record views

39

Files downloads

658