Étude d'un effet répresseur de la forme apo d'ERα dans le contrôle de la prolifération cellulaire

Abstract : Estrogen receptor is the target of classic therapies used against ER+ breast cancers, however, it is often observed that cancerous cells become resistant to these treatments, leading to the dedifferentiation of cancerous cells and the acquisition of a more aggressive phenotype. Some publications suggest that ERα has a repressive effect on proliferation when it is not bonded to its ligand E2. With Western blot and RT-PCR we’ll analyse this repressive effect of ERα and we also suggest with immunostaining how this effect is removed. The removal of this repressive effect allowing the initiation of cells division is correlated to the decrease of ERα concentration in the nucleus when the cells are spreading. This mechanism isn’t due to a phenomenon of dilution but is linked to the activity of RhoGTPase which control tension forces applied on the membrane and on the substrate of the cell.
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Kévin Devidal. Étude d'un effet répresseur de la forme apo d'ERα dans le contrôle de la prolifération cellulaire. Sciences du Vivant [q-bio]. 2018. ⟨dumas-01959220⟩

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