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E. Ancele, Kératites Bactériennes Sévères . Étude Épidémiologique , Clinique et Microbiologique Severe Bacterial Keratitis . A Clinical , Epidemiologic , and Microbiologic Résumé, Journal, 2009.

F. Barría, H. Chabouty, and R. Moreno, Revista Chilena de Infectología Pacientes Que Se Someterán a Cirugía de Cataratas Microbial Flora Isolated From, 2016.

P. Barry, L. Cordov, and S. Gardner, Le Film Lacrymal, 2006.

H. Bennett, J. Hay, C. Kirkness, D. Seal, and P. Devonshire, Antimicrobial Management of Presumed Microbial Keratitis%: Guidelines for Treatment of Central and Peripheral Ulcers, pp.137-182, 1998.

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T. Bourcier, Rapport SFo Surface Oculaire, 2016.

T. Bourcier, . Thomas, C. Borderie, L. Chaumeil, and . Laroche, Bacterial Keratitis%: Predisposing Factors , Clinical and Microbiological Review of 300 Cases, vol.87, pp.834-872, 2003.

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C. Creuzot-garcher, Influence Des Anomalies Du Film Lacrymal Sur La Surface Oculaire, Journal Francais d'Ophtalmologie, vol.29, 2006.

M. A. Dahlgren, A. Lingappan, and K. R. Wilhelmus, The Clinical Diagnosis of Microbial Keratitis, vol.143, pp.940-984, 2008.

A. Darugar, T. Gaujoux, P. Goldschmidt, C. Chaumeil, L. Laroche et al., Caractéristiques Cliniques, Microbiologiques et Thérapeutiques d'une, 2011.

, Série de 111 Kératites Bactériennes Sévères, Journal Français d'Ophtalmologie, vol.34, issue.6, pp.362-68

C. R. Davison, S. J. Tuft, and J. K. Dart, Conjunctival Necrosis after Administration of Topical Fortified Aminoglycosides, American Journal of Ophthalmology, vol.111, issue.6, pp.76771-76777, 1991.

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. Group, Ofloxacin Monotherapy for the Primary Treatment of Microbial Keratitis: A Double-Masked, Randomized, Controlled Trial with Conventional Dual Therapy, Ophthalmology, vol.104, issue.11, pp.1902-1911, 1997.

A. Grzybowski, P. Brona, and S. J. Kim, Microbial Flora and Resistance in Ophthalmology: A Review, Clinical and Experimental Ophthalmology, vol.255, issue.5, pp.851-62, 2017.

G. Gunduz, A. Gunduz, N. Polat, Y. Birgul-elbozan-cumurcu, and . Yakupogullart, The Effect of Chronic Alcoholism on the Conjunctival Flora The Effect of Chronic Alcoholism on the Conjunctival Flora, Current, pp.734-773, 2016.

M. Hanet, J. Sophie, A. P. Jamart, and . Chaves, Fluoroquinolones or Fortified Antibiotics for Treating Bacterial Keratitis: Systematic Review and Meta-Analysis of Comparative Studies, Canadian Journal of Ophthalmology, vol.47, issue.6, pp.493-99, 2012.

N. C. Lim, K. A. Dawn, M. Lim, and . Ray, Polymicrobial versus Monomicrobial Keratitis: A Retrospective Comparative Study, Eye and Contact Lens, vol.39, issue.5, pp.348-54, 2013.

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C. N. Ly, J. N. Pham, P. R. Badenoch, S. M. Bell, G. Hawkins et al., Bacteria Commonly Isolated from Keratitis Specimens Retain Antibiotic Susceptibility to Fluoroquinolones and Gentamicin plus Cephalothin, Clinical and Experimental Ophthalmology, vol.34, issue.1, pp.44-50, 2006.

F. Malet, Rapport de Contactologie SFO, 2009.

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S. Mishima, Clinical Pharmacokinetics of the Eye, Clinical Pharmacokinetics, vol.21, pp.504-545, 1981.

N. Morlet, D. Minassian, J. Butcher, and S. Group, Risk Factors for Treatment Outcome of Suspected Microbial Keratitis, pp.1027-1058, 1999.

S. Ohtani, K. Shimizu, R. Nejima, F. Kagaya, M. Aihara et al., Conjunctival Bacteria Flora of Glaucoma Patients during Long-Term Administration of Prostaglandin Analog Drops, Investigative Ophthalmology and Visual Science, vol.58, issue.10, pp.3991-96, 2017.

E. Osborn, J. Baum, C. Ernst, and P. Koch, The Stability of Ten Antibiotics in Artificial Tear Solutions, American Journal of Ophthalmology, vol.82, issue.5, pp.775-80, 1976.

M. B. Raizman, A. L. Jay-m-rabin, M. Graves, and . Rinehart, Tear Concentrations of Levofloxacin Following Topical Administration of a Single Dose of 0 . 5 % Levofloxacin Ophthalmic Solution in Healthy Volunteers, vol.24, pp.1439-50, 2002.

P. Robert and E. Denes, Antibiothérapie Locale En Ophtalmologie, Ophtalmologie, vol.5, pp.1-17, 2012.

J. W. Shell, Therapeutic Review%: Pharmacokinetics of Topically Applied Ophtalmic Drugs, Survey of Ophtalmology, vol.26, issue.4, 1982.

G. Stern, G. Schemmer, R. Farber, and M. Gorovoy, Effect of Topical Antibiotic Solutions on Corneal Epithelial Wound Healing, Archives of Ophthalmology, vol.101, issue.4, pp.644-691, 1983.

P. Thorn and S. Johansen, Pharmacokinetic Investigation of Fusidic Acid 1%, 1997.

, Viscous Eye Drops in Healthy Volunteers, European Journal of Ophthalmology, vol.7, issue.1, pp.9-12

M. C. Vital, M. Belloso, T. C. Prager, and J. D. Lanier, Classifying the Severity of Corneal Ulcers by Using the '1, 2, 3' Rule, Cornea, vol.26, issue.1, pp.16-20, 2007.

B. C. Weston and J. W. Loveless, Canalicular Stenosis Due to Topical Use of Fortified Antibiotics, Canadian Journal of Ophthalmology, vol.35, issue.6, pp.80062-80071, 2000.

S. Zhang, J. N. Dan, T. He, C. Niu, C. Yeung-chan et al., Bacteriological Profile of Ocular Surface Flora in Meibomian Gland Dysfunction, Ocular Surface, vol.15, issue.2, pp.242-289, 2017.

S. Zhang, J. N. Dan, T. He, S. S. Niu, C. Liu et al., Effectiveness of Meibomian Gland Massage Combined with Topical Levofloxacin against Ocular Surface Flora in Patients before Penetrating Ocular Surgery, The Ocular Surface, 2017.

M. Adam, M. Balc?, A. Hasan, A. Ç. Bayhan, M. ?nkaya et al., Conjunctival Flora in Diabetic and Nondiabetic Individuals, Turk Oftalmoloiji Dergisi, vol.45, issue.5, pp.193-96, 2015.

A. , Collyres Et Autres Topiques Antibiotiques Dans Les Infections Oculaires Superficielles Recommandations Collyres Et Autres Topiques Antibiotiques Dans Les Infections Oculaires Superficielles, vol.10, p.6, 2004.

M. Ai, R. Cj, L. Pr, and C. Ej, Risk Factors in Microbial Keratitis Leading to Penetrating, vol.106, pp.90250-90256, 1999.

C. Allouch-nahmias, . Goldschmit, . Borderie, . Touzeau, . Gaujoux et al., Anatomie de La Cornée, Ophtalmologie, vol.8, issue.3, pp.44155-44156, 2011.

E. Ancele, Kératites Bactériennes Sévères . Étude Épidémiologique , Clinique et Microbiologique Severe Bacterial Keratitis . A Clinical , Epidemiologic , and Microbiologic Résumé, Journal, 2009.

F. Barría, H. Chabouty, and R. Moreno, Revista Chilena de Infectología Pacientes Que Se Someterán a Cirugía de Cataratas Microbial Flora Isolated From, 2016.

C. Baudouin and . Labbé, Le Film Lacrymal, 2006.

H. Bennett, J. Hay, C. Kirkness, D. Seal, and P. Devonshire, Antimicrobial Management of Presumed Microbial Keratitis%: Guidelines for Treatment of Central and Peripheral Ulcers, pp.137-182, 1998.

J. Bernard, P. Ritleng, A. Ducasse, V. Ameline, and F. Mann, Physiologie de l'excrétion Des Larmes : Les Voies Lacrymales, EMC -Ophtalmologie, vol.5, issue.2, pp.1-12, 2008.

T. Bourcier, Rapport SFo Surface Oculaire, 2016.

T. Bourcier, . Thomas, C. Borderie, L. Chaumeil, and . Laroche, Bacterial Keratitis%: Predisposing Factors , Clinical and Microbiological Review of 300 Cases, vol.87, pp.834-872, 2003.

C. Chiquet and J. Romanet, Prescrire Les Collyres Fortifiés, Journal Francais d'Ophtalmologie, 2007.

C. ;. Epilly-trop, Maladies Infectieuses Tropicales, 2016.

M. A. Dahlgren, A. Lingappan, and K. R. Wilhelmus, The Clinical Diagnosis of Microbial Keratitis, vol.143, pp.940-984, 2008.

A. Darugar, T. Gaujoux, P. Goldschmidt, C. Chaumeil, L. Laroche et al., Caractéristiques Cliniques, Microbiologiques et Thérapeutiques d'une, 2011.

, Série de 111 Kératites Bactériennes Sévères, Journal Français d'Ophtalmologie, vol.34, issue.6, pp.362-68

C. R. Davison, S. J. Tuft, and J. K. Dart, Conjunctival Necrosis after Administration of Topical Fortified Aminoglycosides, American Journal of Ophthalmology, vol.111, issue.6, pp.76771-76777, 1991.

D. R. Graham, D. Jeffrey, C. Band, D. G. Thornsberry, and R. E. Hollis,

K. J. Weaver, L. Ray, and . Prajna, Fluoroquinolone Treatment and Susceptibility of Isolates from Bacterial Keratitis, Clinical Infectious Diseases, vol.131, issue.3, pp.310-323, 1990.

. Group, Ofloxacin Monotherapy for the Primary Treatment of Microbial Keratitis: A Double-Masked, Randomized, Controlled Trial with Conventional Dual Therapy, Ophthalmology, vol.104, issue.11, pp.1902-1911, 1997.

A. Grzybowski, P. Brona, and S. J. Kim, Microbial Flora and Resistance in Ophthalmology: A Review, Clinical and Experimental Ophthalmology, vol.255, issue.5, pp.851-62, 2017.

G. Gunduz, A. Gunduz, N. Polat, Y. Birgul-elbozan-cumurcu, and . Yakupogullart, The Effect of Chronic Alcoholism on the Conjunctival Flora The Effect of Chronic Alcoholism on the Conjunctival Flora, Current, pp.734-773, 2016.

M. Hanet, J. Sophie, A. P. Jamart, and . Chaves, Fluoroquinolones or Fortified Antibiotics for Treating Bacterial Keratitis: Systematic Review and Meta-Analysis of Comparative Studies, Canadian Journal of Ophthalmology, vol.47, issue.6, pp.493-99, 2012.

N. C. Lim, K. A. Dawn, M. Lim, and . Ray, Polymicrobial versus Monomicrobial Keratitis: A Retrospective Comparative Study, Eye and Contact Lens, vol.39, issue.5, pp.348-54, 2013.

L. J. Lu and J. Liu, Human Microbiota and Ophthalmic Disease, Yale Journal of Biology and Medicine, vol.89, issue.3, pp.325-355, 2016.

C. N. Ly, J. N. Pham, P. R. Badenoch, S. M. Bell, G. Hawkins et al., Bacteria Commonly Isolated from Keratitis Specimens Retain Antibiotic Susceptibility to Fluoroquinolones and Gentamicin plus Cephalothin, Clinical and Experimental Ophthalmology, vol.34, issue.1, pp.44-50, 2006.

F. Malet, Rapport de Contactologie SFO, 2009.

S. Mcleod, L. D-labree, R. Tayyanipour, C. Flowers, P. Lee et al., The Importance of Initial Management in the Treatment of Severe Infectious Corneal Ulcers, Ophthalmology, vol.102, issue.12, pp.30771-30774, 1995.

S. Mishima, Clinical Pharmacokinetics of the Eye, Clinical Pharmacokinetics, vol.21, pp.504-545, 1981.

N. Morlet, D. Minassian, J. Butcher, and S. Group, Risk Factors for Treatment Outcome of Suspected Microbial Keratitis, pp.1027-1058, 1999.

S. Ohtani, K. Shimizu, R. Nejima, F. Kagaya, M. Aihara et al., Conjunctival Bacteria Flora of Glaucoma Patients during Long-Term Administration of Prostaglandin Analog Drops, Investigative Ophthalmology and Visual Science, vol.58, issue.10, pp.3991-96, 2017.

E. Osborn, J. Baum, C. Ernst, and P. Koch, The Stability of Ten Antibiotics in Artificial Tear Solutions, American Journal of Ophthalmology, vol.82, issue.5, pp.775-80, 1976.

P. Robert and E. Denes, Antibiothérapie Locale En Ophtalmologie, Ophtalmologie, vol.5, pp.1-17, 2012.

G. Stern, G. Schemmer, R. Farber, and M. Gorovoy, Effect of Topical Antibiotic Solutions on Corneal Epithelial Wound Healing, Archives of Ophthalmology, vol.101, issue.4, pp.644-691, 1983.

P. Thorn and S. Johansen, Pharmacokinetic Investigation of Fusidic Acid 1%, 1997.

, Viscous Eye Drops in Healthy Volunteers, European Journal of Ophthalmology, vol.7, issue.1, pp.9-12

M. C. Vital, M. Belloso, T. C. Prager, and J. D. Lanier, Classifying the Severity of Corneal Ulcers by Using the '1, 2, 3' Rule, Cornea, vol.26, issue.1, pp.16-20, 2007.

B. C. Weston and J. W. Loveless, Canalicular Stenosis Due to Topical Use of Fortified Antibiotics, Canadian Journal of Ophthalmology, vol.35, issue.6, pp.80062-80071, 2000.

S. Zhang, J. N. Dan, T. He, C. Niu, C. Yeung-chan et al., Bacteriological Profile of Ocular Surface Flora in Meibomian Gland Dysfunction, Ocular Surface, vol.15, issue.2, pp.242-289, 2017.

S. Zhang, J. N. Dan, T. He, S. S. Niu, C. Liu et al., Conditions : ! Milieu : sur Muller Hinton Boite carré X2 (MHE ou MHF en fonction du germe), un isolement sur gélose TSH ou TSA en fonction du germe afin de garantir la pureté de l'isolat étudié et conserver la souche, 2017.

!. Suspension, Mac Farland 0,5 sans dilution/Solution saline (0,9% de NaCl) ! Atmosphère : aérobie ou CO2

. !-température-d, incubation : 35 ± 2 °C ! Temps d'incubation : 16 -24H (indiquer l'heure d'ensemencement sur la boîte) -prolonger l'incubation si souche déficiente

, Pour TOUT prélèvement d'oeil (cornée, humeur aqueuse, vitré, conjonctive)

S. Abcès-de-cornée, CMI Etest (x8) sur gélose adapté au germe : -Ceftazidime (CZD) -Tobramycine (TM) -Amikacine (AN) -Azithromycine (AZM) -Ciprofloxacine (CIP) -Rifampicine (RA) -Vancomycine (VA) -Acide fusidique (FA)

, « Par délibération de son Conseil en date du 10 Novembre, 1972.