A. Auteurs and .. .. ,

B. .. Résumé,

E. .. Discussion,

, Les cellules souches mésenchymateuses : une option de choix dans le traitement des cordes vocales cicatricielles

, Caractéristiques et valeur ajoutée de la fraction vasculaire stromale

F. .. Conclusion,

G. .. Références,

. Ii, Résultats de l'essai clinique CELLCORDES -Première injection chez l'homme de fraction vasculaire stromale autologue d'origine adipeuse dans des cordes vocales cicatricielles : essai clinique prospectif ouvert de phase I (Article 2)

A. Auteurs and .. .. ,

B. .. Résumé,

C. .. Abréviations,

E. .. Méthodes,

, Conception de l'étude et critères d'éligibilité

, Lipoaspiration et Préparation de la Fraction Vasculaire Stromale

.. .. Protocole-thérapeutique,

.. .. Analyses,

F. .. Résultats,

. Caractéristiques and . .. Fvs,

, Sécurité et tolérance de l'injection autologue de FVS

, Efficacité de l'injection autologue de FVS d'origine adipeuse

G. .. Discussion,

H. .. Remerciements,

I. .. Références,

, Cas clinique détaillé d'une patiente incluse dans l'essai CELLCORDES -Fraction vasculaire stromale d'origine adipeuse et cordes vocales cicatricielles : premier cas clinique (Article 3)

A. Auteurs and .. .. ,

B. .. Résumé,

F. Matériels and .. .. ,

.. .. Patiente,

.. .. Protocole,

G. .. Résultats,

K. .. Références, , p.61

A. Essai,

B. Projet-amadeus and .. .. ,

C. , Quelles perspectives pour l'avenir ?

G. Friedrich, F. G. Dikkers, and C. Arens, Vocal fold scars: current concepts and future directions. Consensus report of the phonosurgery committee of the European laryngological society, European Archives of Oto-Rhino-Laryngology, vol.270, issue.9, pp.2491-507, 2013.

M. Hirano and . Phonosurgery, Basic and clinical investigations, 1975.

M. Gugatschka, T. Kojima, S. Ohno, S. Kanemaru, and S. Hirano,

, Side Population Cells During Wound Healing in Rat Vocal Folds, Laryngoscope, vol.121, issue.8, pp.1662-1669, 2011.

S. Hertegard, A. Dahlqvist, and E. Goodyer, Viscoelastic measurements after vocal fold scarring in rabbits--short-term results after hyaluronan injection, Acta Otolaryngol

G. Molteni, G. Bergamini, and A. Ricci-maccarini, Auto-crosslinked hyaluronan gel injections in phonosurgery, Otolaryngol Head Neck Surg, vol.142, issue.4, pp.547-53, 2010.

A. Friedstein, Osteogenic stem cells in bone marrow, Bone and mineral Research, pp.243-72, 1990.

B. M. Strem, K. C. Hicok, and M. Zhu, Multipotential differentiation of adipose tissuederived stem cells, Keio J Med, vol.54, issue.3, pp.132-173, 2005.

N. Hiwatashi, S. Hirano, and M. Mizuta, Adipose-derived stem cells versus bone marrow-derived stem cells for vocal fold regeneration, Laryngoscope, vol.124, issue.12, pp.461-470, 2014.

N. Hiwatashi, S. Hirano, and R. Suzuki, Comparison of ASCs and BMSCs combined with atelocollagen for vocal fold scar regeneration, Laryngoscope, vol.126, issue.5, pp.1143-50, 2016.

J. Cedervall, L. Ahrlund-richter, and B. Svensson, Injection of embryonic stem cells into scarred rabbit vocal folds enhances healing and improves viscoelasticity: short-term results, Laryngoscope, vol.117, issue.11, pp.2075-81, 2007.

B. Svensson, S. R. Nagubothu, and C. Nord, Stem Cell Therapy in Injured Vocal Folds: A Three-Month Xenograft Analysis of Human Embryonic Stem Cells. Biomed Research International, 2015.

H. Peng, M. L. Yang, and R. , The use of laryngeal mucosa mesenchymal stem cells for the repair the vocal fold injury, Biomaterials, vol.34, issue.36, pp.9026-9061, 2013.

Y. Kumai, J. B. Kobler, and H. Park, Crosstalk between adipose-derived stem/stromal cells and vocal fold fibroblasts in vitro, Laryngoscope, vol.119, issue.4, pp.799-805, 2009.

Y. Kumai, J. B. Kobler, H. Park, M. Galindo, V. L. Herrera et al., Modulation of vocal fold scar fibroblasts by adipose-derived stem/stromal cells, Laryngoscope, vol.120, issue.2, pp.330-337, 2010.

Y. Kishimoto, S. Hirano, and Y. Kitani, Chronic vocal fold scar restoration with hepatocyte growth factor hydrogel, Laryngoscope, vol.120, issue.1, pp.108-121, 2010.

S. Hirano, D. M. Bless, and B. Rousseau, Prevention of vocal fold scarring by topical injection of hepatocyte growth factor in a rabbit model, Laryngoscope, vol.114, issue.3, pp.548-56, 2004.

X. Chen and S. L. Thibeault, Cell-cell interaction between vocal fold fibroblasts and bone marrow mesenchymal stromal cells in three-dimensional hyaluronan hydrogel, Journal of Tissue Engineering and Regenerative Medicine, vol.10, issue.5, pp.437-483, 2016.

N. Hiwatashi, R. Bing, I. Kraja, and R. C. Branski, Mesenchymal stem cells have antifibrotic effects on transforming growth factor-?1-stimulated vocal fold fibroblasts, Laryngoscope

B. Svensson, S. R. Nagubothu, and J. Cedervall, Injection of human mesenchymal stem cells improves healing of vocal folds after scar excision--a xenograft analysis, Laryngoscope, vol.121, issue.10, pp.2185-90, 2011.

B. Svensson, R. S. Nagubothu, and J. Cedervall, Injection of human mesenchymal stem cells improves healing of scarred vocal folds: analysis using a xenograft model, Laryngoscope, vol.120, issue.7, pp.1370-1375, 2010.

G. Friedrich, F. G. Dikkers, C. Arens, M. Remacle, M. Hess et al., Vocal fold scars: current concepts and future directions. Consensus report of the phonosurgery committee of the European laryngological society, Eur Arch Otorhinolaryngol, vol.270, issue.9, pp.2491-507, 2013.

M. Hirano, Phonosurgery: basic and clinical investigation. Otologia (Fukuoka), vol.21, pp.239-440, 1975.

M. Graupp, S. Bachna-rotter, C. Gerstenberger, G. Friedrich, E. Fröhlich-sorger et al., The unsolved chapter of vocal fold scars and how tissue engineering could help us solve the problem, Eur Arch Otorhinolaryngol. sept, vol.273, issue.9, pp.2279-84, 2016.

R. T. Sataloff, J. R. Spiegel, R. J. Heuer, M. M. Baroody, K. A. Emerich et al., Laryngeal minimicroflap: a new technique and reassessment of the microflap saga, J Voice, vol.9, issue.2, pp.198-204, 1995.

N. V. Welham, S. H. Choi, S. H. Dailey, C. N. Ford, J. J. Jiang et al., Prospective multi-arm evaluation of surgical treatments for vocal fold scar and pathologic sulcus vocalis

, Laryngoscope, vol.121, issue.6, pp.1252-60, 2011.

G. Molteni, G. Bergamini, A. Ricci-maccarini, C. Marchese, A. Ghidini et al., Auto-crosslinked hyaluronan gel injections in phonosurgery, Otolaryngol Head Neck Surg, vol.142, issue.4, pp.547-53, 2010.

S. Hertegard, L. Hallen, C. Laurent, E. Lindström, K. Olofsson et al., Cross-linked hyaluronan versus collagen for injection treatment of glottal insufficiency: 2-year followup, Acta Otolaryngol, vol.124, pp.1208-1214, 2004.

Y. Liu, Z. Shu, X. Prestwich, and G. D. , Biocompatibility and stability of disulfidecrosslinked hyaluronan films, Biomaterials, vol.26, issue.23, pp.4737-4783, 2005.

Y. Zhang, F. Rossi, S. Papa, M. B. Violatto, P. Bigini et al., Non-invasive in vitro and in vivo monitoring of degradation of fluorescently labeled hyaluronan hydrogels for tissue engineering applications, Acta Biomater, vol.30, pp.188-98, 2016.

J. Magalon, M. Velier, S. Simoncini, P. François, B. Bertrand et al., Molecular profile and proangiogenic activity of the adipose-derived stromal vascular fraction used as an autologous innovative medicinal product in patients with systemic sclerosis, Ann Rheum Dis, 2019.

J. A. Dykstra, T. Facile, R. J. Patrick, K. R. Francis, S. Milanovich et al., Concise Review: Fat and Furious: Harnessing the Full Potential of Adipose-Derived Stromal Vascular Fraction. Stem Cells Transl Med, vol.6, pp.1096-1108, 2017.

Y. Kumai, J. B. Kobler, H. Park, G. Lopez-guerra, S. Karajanagi et al., Crosstalk between adipose-derived stem/stromal cells and vocal fold fibroblasts in vitro, Laryngoscope, vol.119, pp.799-805, 2009.

Y. Kumai, J. B. Kobler, H. Park, M. Galindo, V. L. Herrera et al., Modulation of vocal fold scar fibroblasts by adipose-derived stem/stromal cells, Laryngoscope, vol.120, pp.330-337, 2010.

A. Mattei, J. Magalon, B. Bertrand, C. Philandrianos, J. Veran et al., Cell therapy and scarred vocal folds. Eur Ann Otorhinolaryngol Head Neck Dis, pp.30103-30108, 2017.

N. Hiwatashi, S. Hirano, and M. Mizuta, Adipose-derived stem cells versus bone marrowderived stem cells for vocal fold regeneration, Laryngoscope, vol.124, pp.461-470, 2014.

N. Hiwatashi, S. Hirano, R. Suzuki, Y. Kawai, M. Mizuta et al., Comparison of ASCs and BMSCs combined with atelocollagen for vocal fold scar regeneration

, Laryngoscope, vol.126, pp.1143-50, 2016.

W. Xu, R. Hu, F. E. Han, and D. , Adipose-derived mesenchymal stem cells in collagenhyaluronic acid gel composite scaffolds for vocal fold regeneration, Ann OtolRhinol Laryngol, vol.120, issue.2, pp.123-153, 2011.

Y. M. Kim, S. H. Oh, J. S. Choi, S. Lee, J. C. Ra et al., Adipose-derived stem cellcontaining hyaluronicacid/alginate hydrogel improves vocal fold wound healing

, Laryngoscope, vol.124, issue.3, pp.64-72, 2014.

S. K. Kwon and B. J. Lee, The combined effect of autologous mesenchymal stem cells and hepatocyte growth factor on vocal fold regeneration and fibrosis in vocal fold wound, J Tissue Eng Regen Med, vol.5, issue.4-6, pp.735-777, 2008.

T. L. Shiba, J. Hardy, G. Luegmair, Z. Y. Zhang, and J. L. Long, Tissue-engineeredvocal fold mucosa implantation in rabbits, Otolaryngol Head Neck, vol.154, issue.4, pp.679-88, 2016.

V. Angelou, V. Kalodimou, I. Messini, N. Psychalakis, K. Eleftheria et al., Adiposederived mesenchymal stem cells in the regeneration of vocal folds:a study on a chronic vocal fold scar, Stem Cells Int, p.9010279, 2016.

G. De-bonnecaze, B. Chaput, V. Woisard, E. Uro-coste, P. Swider et al., Adipose stromal cells improve healing of vocal fold scar: morphological and functional evidences

, Laryngoscope, vol.126, issue.8, pp.278-85, 2016.

S. J. Hong, S. H. Lee, S. M. Jin, S. Y. Kwon, K. Y. Jung et al., Vocal fold wound healing after injection of human adipose-derived stem cells in a rabbit model, Acta Otolaryngol, vol.131, issue.11, pp.1198-204, 2011.

R. Hu, W. Ling, W. Xu, and D. Han, Fibroblast-like cells differentiated from adipose-derived mesenchymal stem cells for vocal fold wound healing, PLoS One, vol.9, issue.3, p.92676, 2014.

B. J. Lee, S. G. Wang, J. C. Lee, J. S. Jung, Y. C. Bae et al., The prevention of vocal fold scarring using autologous adipose tissue-derived stromal cells, Cells Tissues Organs, vol.184, issue.3-4, pp.198-204, 2006.

S. Atalay, A. Coruh, and K. Deniz, Stromal vascular fraction improves deep partial thickness burn wound healing, Burns, vol.40, issue.7, pp.1375-83, 2014.

A. Bura, V. Planat-benard, P. Bourin, J. S. Silvestre, F. Gross et al., Phase I triaG : the use of autologous cultured adipose-derived stroma/stem cells to treat patients with non-revascularizable critical limb ischemia, Cytotherapy, vol.16, issue.2, pp.245-57, 2014.

A. J. Leblanc, J. S. Touroo, J. B. Hoying, and S. K. Williams, Adipose stromal vascular fraction cell construct sustains coronary microvascular function after acute myocardial infarction, Am J Physiol Heart Circ Physiol, vol.302, issue.4, pp.973-82, 2012.

S. Hashmi, M. Ahmed, M. H. Murad, M. R. Litzow, R. H. Adams et al., Survival after mesenchymal stromal cell therapy in steroid-refractory acute graft-versus-host disease: systematic review and meta-analysis, Lancet Haematol, vol.3, issue.1, pp.45-52, 2016.

B. Granel, A. Daumas, E. Jouve, J. R. Harlé, P. S. Nguyen et al., Safety, tolerability and potential efficacy of injection of autologous adipose-derived stromal vascular fraction in the fingers of patients with systemic sclerosis: an open-label phase I trial, Annals of the Rheumatic Diseases, vol.74, issue.12, pp.2175-82, 2015.

M. Karbiener, B. Darnhofer, M. T. Frisch, B. Rinner, R. Birner-gruenberger et al., Comparative proteomics of paired vocal fold and oral mucosa fibroblasts, J Proteomics, vol.155, pp.11-21, 2017.

A. Mattei, J. Magalon, B. Bertrand, F. Grimaud, J. Revis et al., Autologous adiposederived stromal vascular fraction and scarred vocal folds: first clinical case report, Stem Cell Res Ther, vol.9, issue.1, p.202, 2018.
URL : https://hal.archives-ouvertes.fr/hal-02102709

P. Bourin, B. A. Bunnell, L. Casteilla, M. Dominici, A. J. Katz et al., Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: a joint statement of the International Federation for

, Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT), vol.15, pp.641-649, 2013.

P. H. Dejonckere, P. Bradley, P. Clemente, G. Cornut, L. Crevier-buchman et al., A basic protocol for functional assessment of voice pathology, especially for investigating the efficacy of (phonosurgical) treatments and evaluating new assessment techniques. Guideline elaborated by the Committee on Phoniatrics of the European Laryngological Society (ELS), Eur Arch Otorhinolaryngol, vol.258, issue.2, pp.77-82, 2001.

M. Hirano, Psycho-acoustic evaluation of voice: GRBAS Scale for evaluating the hoarse voice, vol.1981, pp.81-84

B. Jacobson, A. Johnson, C. Grywalski, A. Silbergleit, G. Jacobson et al., The Voice Handicap Index (VHI): development and validation, Am J Speech Lang Pathol, vol.6, pp.66-70, 1997.

J. E. Ware, M. Kosinski, and B. Gandek, SF-36 ® Health Survey: Manual & Interpretation Guide

R. I. Lincoln, QualityMetric Incorporated, 1993.

J. E. Ware, M. Kosinski, and S. D. Keller, SF-36 Physical and Mental Health Summary Scales: A User's Manual. Boston, MA: The Health Institute, 1994.

A. Daumas, J. Magalon, E. Jouve, R. Truillet, D. Casanova et al., Long-term follow-up after autologous adipose-derived stromal vascular fraction injection into fingers in systemic sclerosis patients, Current Research in Translational Medicine, vol.65, issue.1, pp.40-43, 2017.
URL : https://hal.archives-ouvertes.fr/hal-01792172

P. Guillaume-jugnot, A. Daumas, J. Magalon, E. Jouve, P. Nguyen et al., Autologous adipose-derived stromal vascular fraction in patients with systemic sclerosis: 12-month follow-up, Rheumatology (Oxford), vol.55, issue.2, pp.301-307, 2016.
URL : https://hal.archives-ouvertes.fr/hal-01452751

A. Mattei, G. Desuter, M. Roux, B. Lee, M. Louges et al., International consensus (ICON) on basic voice assessment for unilateral vocal fold paralysis, Eur Ann Otorhinolaryngol Head Neck Dis, vol.135, issue.1S, pp.11-16, 2018.
URL : https://hal.archives-ouvertes.fr/hal-02102684

W. Young, M. R. Hoffman, I. J. Koszewski, C. W. Whited, B. N. Ruel et al., Voice Outcomes following a Single Office-Based Steroid Injection for Vocal Fold Scar

, Otolaryngol Head Neck Surg, vol.155, pp.820-828, 2016.

M. F. Pittenger, A. M. Mackay, S. C. Beck, R. K. Jaiswal, R. Douglas et al., Multilineage potential of adult human mesenchymal stem cells, Science, vol.284, issue.5411, pp.143-150, 1999.

B. Svensson, S. R. Nagubothu, C. Nord, J. Cedervall, I. Hultman et al., Stem cell therapy in injured vocal folds:a three-month xenograft analysis of human embryonic stem cells, Biomed ResInt, p.754876, 2015.

S. Hertegard, J. Cedervall, B. Svensson, K. Forsberg, F. H. Maurer et al., Viscoelastic and histologic propertiesin scarred rabbit vocal folds after mesenchymal stem cell injection, Laryngoscope, vol.116, pp.1248-54, 2006.

R. Rohban and T. R. Pieber, Mesenchymal Stem and Progenitor Cells in Regeneration: Tissue Specificity and regenerative Potential, Stem Cells Int, p.5173732, 2017.

F. J. Vizoso, N. Eiro, S. Cid, J. Schneider, and R. Perez-fernandez, Mesenchymal Stem Cell Secretome: Toward Cell-Free Therapeutic Strategies in Regenerative Medicine, Int J Mol Sci, vol.18, issue.9, 2017.

O. K. Ma and K. H. Chan, Immunomodulation by mesenchymal stem cells: Interplay between mesenchymal stem cells and regulatory lymphocytes, World J Stem Cells, vol.8, issue.9, pp.268-78, 2016.

N. Li and J. Hua, Interactions between mesenchymal stem cells and the immune system, Cell Mol Life Sci, vol.74, issue.13, pp.2345-2360, 2017.

Y. Song, C. Peng, S. Lv, J. Cheng, S. Liu et al., Adipose-derived stem cells ameliorate renal interstitial fibrosis through inhibition of EMT and inflammatory response via TGF-?1 signaling pathway, Int Immunopharmacol, vol.44, pp.115-122, 2017.

N. Hiwatashi, R. Bing, I. Kraja, and R. C. Branski, Mesenchymal stem cells have antifibrotic effects on transforming growth factor-?1-stimulated vocal fold fibroblasts, Laryngoscope, vol.127, pp.35-41, 2017.

Y. Hirose, Y. Funahashi, Y. Matsukawa, T. Majima, M. Yamaguchi et al., Comparison of trophic factors secreted from human adipose-derived stromal vascular fraction with those from adipose-derived stromal/stem cells in the same individuals

, Cytotherapy, vol.20, issue.4, pp.589-591, 2018.

T. A. Wynn and T. R. Ramalingam, Mechanisms of fibrosis: therapeutic translation for fibrotic disease, Nat Med. 6 juill, vol.18, issue.7, pp.1028-1068, 2012.

S. Domergue, C. Bony, M. Maumus, K. Toupet, E. Frouin et al., Comparison between Stromal Vascular Fraction and Adipose Mesenchymal Stem Cells in Remodeling Hypertrophic Scars, PLoS One, vol.11, issue.5, p.156161, 2016.
URL : https://hal.archives-ouvertes.fr/hal-01834377

J. Données and . Tableau, Effets de l'injection de FVS autologue d'origine adipeuse sur l'auto-évaluation, la videolaryngostroboscopie et les analyses perceptive

, Sélection des cellules viables sur la base de l'expression négative de NucBlue et de la dispersion selon la taille

, CD146 -/ CD34 élevé sont des cellules stromales, CD146 élevé / CD34 moyen rassemblent des péricytes et des cellules transitionnelles ; (d) Les péricytes et les cellules transitionnelles sont divisés en fonction de l'expression de CD90 (+ pour les cellules transitionnelles etpour les péricytes) ; (e) Confirmation de l'identification des cellules stromales sur la base de l'expression de CD90 (+ pour les cellules stromales) ; (f) Parmi les cellules hématopoïétiques, les macrophages sont identifiés sur la base de l'expression de CD14 et de la dispersion en fonction de la taille ; (g) Parmi les cellules CD14 -/ CD45 +, les granulocytes et les lymphocytes sont séparés en fonction de la dispersion selon la taille, Les cellules viables sont divisées en cellules CD45+ (hématopoïétiques) et CD45-(régénératives) ; (c) Parmi les cellules régénératives, trois sous-populations sont identifiées sur la base de l'expression de CD146 et CD34 : CD146 élevé / CD34 élevé sont des cellules endothéliales

, La seconde intervention consistait en l'injection de FVS sous anesthésie générale. La suspension de FVS était injectée à l'aide d'une aiguille de 14G dans le tiers moyen des deux CV cicatricielles (0,45 ml par CV)

, La patiente a pu retourner à son domicile le lendemain avec un traitement antibiotique

, La vidéolaryngostroboscopie permet d'évaluer la fermeture glottique, l'onde muqueuse, sa régularité et sa symétrie. L'auto-évaluation était réalisée à l'aide du Voice Handicap Index

L. Hirano, G pour global, R pour raucité, B pour« breathiness » du signal vocal (plus le signal est haut, meilleur il est). Le temps maximum de phonation (TMP) et le débit d'air buccal (DAB) quantifient la fuite d'air glottique : si la fuite est importante

, Conflits d'intérêt : Les auteurs déclarent ne pas avoir de conflit d

, Assistance Publique des Hôpitaux de Marseille, avec un financement de la Fondation de l'Avenir pour la Recherche Médicale Appliquée, Contributions des auteurs : AM et JM ont contribué de manière égale à la rédaction du manuscrit et à la collecte des données

J. R. Bb and J. V. Et, AG ont grandement contribué à la collecte de données et à la rédaction du manuscrit

F. S. Mv,

K. Références,

G. Friedrich, F. G. Dikkers, C. Arens, M. Remacle, M. Hess et al., Vocal fold scars: current concepts and future directions. Consensus report of the phonosurgery committee of the European laryngological society, Eur Arch Otorhinolaryngol, vol.270, issue.9, pp.2491-507, 2013.

M. Hirano, Phonosurgery: basic and clinical investigation. Otologia (Fukuoka), vol.21, pp.239-440, 1975.

J. A. Dykstra, T. Facile, and R. J. Patrick, Concise Review: Fat and Furious: Harnessing the Full Potential of Adipose-Derived Stromal Vascular Fraction, Stem Cells Transl Med, 2017.

P. Bourin, B. A. Bunnell, L. Casteilla, M. Dominici, A. J. Katz et al., Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissuederived stromal/stem cells: a joint statement of the International Federation for, Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT)

, Cytotherapy, vol.15, issue.6, pp.641-649, 2013.

B. Jacobson, A. Johnson, C. Grywalski, A. Silbergleit, G. Jacobson et al.,

, The Voice Handicap Index (VHI): Development and Validation, Am J Speech Lang Pathol, vol.6, pp.66-70, 1997.

M. Hirano, Psycho-acoustic evaluation of voice: GRBAS Scale for evaluating the hoarse voice, Clinical Examination of voice, 1981.

P. H. Dejonckere, P. Bradley, P. Clemente, G. Cornut, L. Crevier-buchman et al., A basic protocol for functional assessment of voice pathology, especially for investigating the efficacy of (phonosurgical) treatments and evaluating new assessment techniques

, Guideline elaborated by the Committee on Phoniatrics of the European Laryngological Society (ELS), Eur Arch Otorhinolaryngol, vol.258, issue.2, pp.77-82, 2001.

A. J. Leblanc, J. S. Touroo, J. B. Hoying, and S. K. Williams, Adipose stromal vascular fraction

, J Physiol Heart Circ Physiol, vol.302, issue.4, pp.973-82, 2012.

A. Daumas, J. Magalon, E. Jouve, R. Truillet, D. Casanova et al., Long-term follow-up after autologous adipose-derived stromal vascular fraction injection into fingers in systemic sclerosis patients, Curr Res Transl Med, vol.65, issue.1, pp.40-43, 2017.
URL : https://hal.archives-ouvertes.fr/hal-01792172

A. Nguyen, J. Guo, D. A. Banyard, D. Fadavi, J. D. Toranto et al., Stromal vascular fraction: A regenerative reality? Part 1: Current concepts and review of the literature, J Plast Reconstr Aesthet Surg, vol.69, issue.2, pp.170-179, 2016.

S. Atalay, A. Coruh, and K. Deniz, Stromal vascular fraction improves deep partial thickness burn wound healing, Burns, vol.40, issue.7, pp.1375-83, 2014.

A. Mattei, J. Magalon, B. Bertrand, C. Philandrianos, J. Veran et al., Cell therapy and vocal fold scarring, Eur Ann Otorhinolaryngol Head Neck Dis, vol.134, issue.5, pp.339-345, 2017.

J. M. Fishman, J. Long, M. Gugatschka, D. Coppi, P. Hirano et al., Stem cell approaches for vocal fold regeneration, Laryngoscope, vol.126, issue.8, pp.1865-70, 2016.

N. Serratrice, L. Bruzzese, J. Magalon, J. Véran, L. Giraudo et al.,

S. Domergue, C. Bony, M. Maumus, K. Toupet, E. Frouin et al., Comparison between Stromal Vascular Fraction and Adipose Mesenchymal Stem Cells in Remodeling Hypertrophic Scars, ):e0156161. Nous avons dans notre essai CELLCORDES choisi de tester son efficacité dans diverses séquelles carcinologiques, chirurgicales ou post-radiques : cicatrice de cordectomie laser, vol.11, 2016.
URL : https://hal.archives-ouvertes.fr/hal-01834377

. Enfin, utilisation de FVS à visée préventive pourrait également être envisagée immédiatement après chirurgie afin de diminuer le risque d'évolution cicatricielle défavorable, de manière systématique ou par sélection des patients à risque sur des critères cliniques

. C'est, donc avec impatience que nous attendons les résultats des études suscitées qui viendront, nous l'espérons, confirmer et expliquer les propriétés pressenties de la FVS

M. Graupp, H. J. Gruber, G. Weiss, K. Kiesler, S. Bachna-rotter et al.,

M. , Establishing Principles of Macromolecular Crowding for In Vitro Fibrosis Research of the Vocal Fold Lamina Propria, Laryngoscope, vol.125, pp.203-209, 2015.

P. Kumar, A. Satyam, X. Fan, C. E. Rochev, Y. Rodriguez et al., Molecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies, Sci. Rep, 2015.

S. Mazur, A. Zo?oci?ska, K. Siennicka, K. Janik-kosacka, A. Chrapusta et al., Safety of adipose-derived cell (stromal vascular fraction -SVF) augmentation for surgical breast reconstruction in cancer patients, Adv Clin Exp Med, vol.27, issue.8, pp.1085-1090, 2018.

S. Ito, Y. Kai, T. Masuda, F. Tanaka, T. Matsumoto et al.,

C. Calabrese, A. Kothari, S. Badylak, D. Taranto, G. Marcasciano et al., sparing mastectomy: long-term results of a prospective study, Eur Rev Med Pharmacol Sci, vol.22, issue.15, pp.4768-4777, 2018.