Development of analytical methods for the quantification of amoxicillin in human plasma and for the determination of Minimal Biofilm Eradication Concentration and Biofilm Prevention Concentration

Abstract : High doses (>4g/day) of amoxicillin for a long time period (>2 weeks) is currently indicated in the treatment of Bone and Joint Infections (BJI) and Infective Endocarditis (IE) caused by susceptible bacteria in order to be active on planktonic bacteria and biofilm. We performed an observational study in the University Hospital of Grenoble to determine if the plasma concentrations were active on planktonic bacteria and if they could eradicate or prevent the biofilm formation. Between July 2017 and February 2019, 20 patients for whom 22 amoxicillin susceptible bacteria were isolated, were included. LC-MS/MS amoxicillin dosage method was developed according to ISO 15189 standard. Minimal Inhibitory Concentration (MIC), Minimal Biofilm Eradication Concentration (MBEC) and Biofilm Prevention Concentration (BPC) were determined thanks to the “Calgary Biofilm Device”. Plasma concentrations measured were highly variable (<5mg/L to 126,4 mg/L). Based on determined MBEC, amoxicillin concentrations are not sufficient in monotherapy to eradicate E. faecalis biofilm but can eradicate C. acnes biofilm. These in vitro results seem relevant as 5/5 C. acnes infections were cured and 2/5 E. faecalis infections were not. Regarding Streptococci, results depend on the species. Six patients underwent surgery before starting treatment. So, it seems interesting to correlated patients’ outcome with BPC (in process of determination).
Document type :
Master Thesis
Complete list of metadatas

Cited literature [119 references]  Display  Hide  Download

https://dumas.ccsd.cnrs.fr/dumas-02193016
Contributor : Jean-Hugues Morneau <>
Submitted on : Wednesday, July 24, 2019 - 11:10:15 AM
Last modification on : Saturday, July 27, 2019 - 1:18:47 AM

File

2019GREA7032_reynaud_celine(1)...
Files produced by the author(s)

Identifiers

  • HAL Id : dumas-02193016, version 1

Citation

Céline Reynaud. Development of analytical methods for the quantification of amoxicillin in human plasma and for the determination of Minimal Biofilm Eradication Concentration and Biofilm Prevention Concentration. Pharmaceutical sciences. 2019. ⟨dumas-02193016⟩

Share

Metrics

Record views

32

Files downloads

3