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Master Thesis

Effet de la stimulation électroconvulsive sur la neurogenèse adulte hippocampique sur le modèle animal de dépression CORT

Abstract : Introduction: In Major depressive disorder, the electroconvulsive therapy (ECT) is recognized as an effective treatment to face drug-resistance or severe major depression. Although the therapeutic efficacy of ECT is internationally recognized, the neurobiological mechanisms underlying the curative action of ECT remain incompletely understood. Direct access of specific markers in human is not easy, prompting the development of the animal counterpart of ECT, the electro convulsive stimulations (ECS). ECS increases hippocampal progenitor proliferation but these new progenitors have yet to be directly linked to behavioral improvement, because they need 4 weeks to integrate the network. The objective of this project was to analyze the biological effects of ECS treatment on the newborn neuron present before ECS treatment, in a neuroendocrine model of depression, the CORT model. Method: 21 mice received corticosterone treatment (35 g/ml/d) in the drinking water g/ml/d) in the drinking water g/ml/d) in the drinking water g/ml/d) in the drinking water g/ml/d) in the drinking water g/ml/d) in the drinking water g/ml/d) in the drinking water g/ml/d) in the drinking water g/ml/d) in the drinking water g/ml/d) in the drinking water g/ml/d) in the drinking water during 8 weeks. 11 mice have been treated by ECS (10 stimulations under anesthesia, over 2 weeks), while the remainder formed a control group (SHAM). To study post-ECS newborn neurons, mice were injected with EdU (50 mg/kg) once daily for 4 days, 2 weeks before starting the ECS treatment. Results: The number of EdU-positive cells per hippocamp, corresponding to the set of newborn neurons, is significantly higher in the ECS group (median : 676 ; min-max : 478-826) than in the SHAM group (median : 285 ; min-max : 236-368) with p<0,01 (Unilateral Mann-Whitney test). Conclusion: Our work provide evidence that ECS treatment, when applied to CORT mice, increases the survival of hippocampal new-born neurons. Beneficial effect on behavioral traits could be linked to increased survival of neurons present before ECS treatment rather than to the increased number of progenitors following ECS.
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https://dumas.ccsd.cnrs.fr/dumas-02292775
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Submitted on : Friday, September 20, 2019 - 11:04:42 AM
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Anne-Sophie Nguon. Effet de la stimulation électroconvulsive sur la neurogenèse adulte hippocampique sur le modèle animal de dépression CORT. Médecine humaine et pathologie. 2019. ⟨dumas-02292775⟩

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