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, Cependant, l'association entre l'activité du FT sur les MV (activité MV-FT) et la thrombose est limitée par de multiples mécanismes de thrombose liée aux cancers, un rôle plus complexe des MV dans la thrombose et enfin des limites technologiques dans le test mesurant l'activité MV FT

, L'amélioration du dosage de l'activité du FT a été réalisée en optimisant i/la vitesse et le temps de centrifugation, ii/l'utilisation d'un puissant anticorps anti-FT inhibiteur iii/l'utilisation de FVII et d'un substrat fluorogène pour augmenter la spécificité. La spécificité de mesure de l'activité MV-FT a été démontrée par l'absence d'activité sur une lignée de cellules qui expriment le FT en utilisant un anticorps monoclonal anti-FT appelé SBTF-1, qui présente un effet inhibiteur de FT supérieur à celui de l'anticorps monoclonal anti-FT humain, le plus utilisé dans la littérature, appelé HTF-1. Des expériences utilisant du sang provenant d'individus en bonne santé, stimulés ou non par le LPS, ou de plasma enrichi avec 3 niveaux différents de MV ont montré que le nouveau dosage était plus sensible et détecte de l'activité du FT dans les échantillons de plasma sans plaquettes (PFP) provenant d'individus sains. Cependant, le test a été limité par une variabilité intertest, principalement due à l'étape de centrifugation. Ainsi, nous avons amélioré la sensibilité de l'activité MV-FT sans perdre de spécificité, Plusieurs méthodes ont été décrites pour mesurer l'activité MV-FT, mais elles sont gênées par une sensibilité et une spécificité limitées. L'objectif est d'augmenter la sensibilité de l'analyse de l'activité de MV-FT en comparaison à un test préexistant (appelé test de Chapel Hill