Skip to Main content Skip to Navigation
Master Thesis

Characterization of two somaclonal lines of cucumber (Cucumis sativus L.) through a transcriptomic approach

Abstract : Cucumber has a high economic value in the world and particularly in eastern countries in Europe. It was already widely studied, giving tools for the breeders to create new varieties. Incidentally, NGS made plant breeding way easier. Different sources of nex plant material are available. Among them, somaclonal variation (mutations caused by in vitro culture) can be found. They are the target of this study. Two cucumber somaclonal lines, S2 and S3, were studied. Both phenotypes were compared. An RNA-seq analysis was carried out to determine DEG between somaclonal lines and the wild type from which they derive, B10. Some DEGs were checked by qPCR with an average success of 97%. A protein network was built with the DEGs as well as chromosome maps and heatmaps. A promoter analysis was carried out. The conclusions of the analyses suggest that the observed delay of growth comes from genes related to cell cycle, hormones and transcription factors, under the control of diverse promoter elements implied in developmental processes. The delay of flowering can be explained by genes related to hormones and transcription factors under the influence of hormones, light and the circadian cycle. However, nothing was identified to explain the difference of color of S3 or the supposed difference between S2 and B10 fruit.
Document type :
Master Thesis
Complete list of metadatas

Cited literature [110 references]  Display  Hide  Download

https://dumas.ccsd.cnrs.fr/dumas-02395983
Contributor : Agrocampus Ouest <>
Submitted on : Thursday, December 5, 2019 - 5:35:04 PM
Last modification on : Monday, July 6, 2020 - 3:38:27 PM
Long-term archiving on: : Friday, March 6, 2020 - 7:37:59 PM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : 2024-01-15

Please log in to resquest access to the document

Licence


Distributed under a Creative Commons Attribution - NonCommercial - NoDerivatives 4.0 International License

Identifiers

  • HAL Id : dumas-02395983, version 1

Collections

Citation

Estelle Bystrzycki. Characterization of two somaclonal lines of cucumber (Cucumis sativus L.) through a transcriptomic approach. Life Sciences [q-bio]. 2019. ⟨dumas-02395983⟩

Share

Metrics

Record views

20