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Master Thesis

Characterization of two somaclonal lines of cucumber (Cucumis sativus L.) through a transcriptomic approach

Abstract : Cucumber has a high economic value in the world and particularly in eastern countries in Europe. It was already widely studied, giving tools for the breeders to create new varieties. Incidentally, NGS made plant breeding way easier. Different sources of nex plant material are available. Among them, somaclonal variation (mutations caused by in vitro culture) can be found. They are the target of this study. Two cucumber somaclonal lines, S2 and S3, were studied. Both phenotypes were compared. An RNA-seq analysis was carried out to determine DEG between somaclonal lines and the wild type from which they derive, B10. Some DEGs were checked by qPCR with an average success of 97%. A protein network was built with the DEGs as well as chromosome maps and heatmaps. A promoter analysis was carried out. The conclusions of the analyses suggest that the observed delay of growth comes from genes related to cell cycle, hormones and transcription factors, under the control of diverse promoter elements implied in developmental processes. The delay of flowering can be explained by genes related to hormones and transcription factors under the influence of hormones, light and the circadian cycle. However, nothing was identified to explain the difference of color of S3 or the supposed difference between S2 and B10 fruit.
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Master Thesis
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Submitted on : Thursday, December 5, 2019 - 5:35:04 PM
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  • HAL Id : dumas-02395983, version 1




Estelle Bystrzycki. Characterization of two somaclonal lines of cucumber (Cucumis sativus L.) through a transcriptomic approach. Life Sciences [q-bio]. 2019. ⟨dumas-02395983⟩



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