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Master Thesis

Validation de méthode de la trousse EurobioPlex qRT-PCR Hépatite Delta au laboratoire de virologie du CHU de Bordeaux

Abstract : Hepatitis Delta virus (HDV) is a satellite of Hepatitis B virus (HBV). Its prevalence is underestimated, but 10 to 20 millions persons could be infected all around the world. When HBV-HDV coinfection is serologically diagnosed, HDV RNA must be quantified to confirm its replicative status and insure a proper follow up of the patients. This is particularly important because anti anti-viral treatment (Bulevirtide) is now available. According to our aims, our study allowed the biological validation of the commercially available quantitative RT-PCR assay EurobioPlex qRT-PCR HDV in Bordeaux University Hospital Virology department. We compared our results on 23 clinical samples with those obtained by the National Reference Center (CNR) for HDV infection. Results were expressed in IU/mL thanks to a WHO international standard. Our quantitative results appeared underestimated in comparison with those obtained by the CNR on the same samples ; this could result from the use of different extraction and amplification systems. However, correlation, concordance and conformity data were satisfactory. This allowed us to submit this method in our laboratory setting to the national external quality evaluation. Hopefully, the test will soon be used in the laboratory for the diagnosis and monitoring of HDV-positive patients.
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https://dumas.ccsd.cnrs.fr/dumas-02864375
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Submitted on : Thursday, June 11, 2020 - 9:27:59 AM
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Tania-Stefana Mihailescu. Validation de méthode de la trousse EurobioPlex qRT-PCR Hépatite Delta au laboratoire de virologie du CHU de Bordeaux. Sciences du Vivant [q-bio]. 2020. ⟨dumas-02864375⟩

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