P. Kavirayani-indira, The Chemistry of Curcumin: From Extraction to Therapeutic Agent, Molecules, vol.19, pp.20091-20112, 2014.

P. N. Ravindran, K. Babu, and K. Sivaraman, Turmeric: the genus Curcuma. Medicinal and aromatic plants--industrial profiles, 2007.

P. Scartezzini and E. Speroni, Review on some plants of Indian traditional medicine with antioxidant activity, J Ethnopharmacol. juill, vol.71, issue.1-2, pp.23-43, 2000.

H. P. Ammon and M. A. Wahl, Pharmacology of Curcuma longa, Planta Med, vol.57, issue.1, pp.1-7, 1991.

G. K. Jayaprakasha, J. Mohan-rao, L. Sakariah, and K. K. , Chemistry and biological activities of C. longa, déc, vol.16, pp.533-548, 2005.

, Curcuma longa L. 1753 [Internet

, World Health Organization Geneva. WHO monographs on selected medicinal plants. World Health Organization Geneva, 1999.

A. Goel, A. B. Kunnumakkara, and B. B. Aggarwal, Curcumin as "Curecumin": From kitchen to clinic, Biochem Pharmacol, vol.75, issue.4, pp.787-809, 200815.

F. P. Chaumeton and . Flore-médicale, , vol.7, p.1816

A. and P. Française, , 2017.

T. Esatbeyoglu, P. Huebbe, I. M. Ernst, D. Chin, A. E. Wagner et al., Curcuminfrom molecule to biological function, Angew Chem Int Ed Engl, vol.51, issue.22, pp.5308-5340, 2012.

J. H. Lee and M. G. Choung, Determination of curcuminoid colouring principles in commercial foods by HPLC. Food Chem, vol.124, pp.1217-1239, 2011.

M. Scotter, Synthesis and chemical characterisation of curcuminoid colouring principles for their potential use as HPLC standards for the determination of curcumin colour in foods. LWT -Food Sci Technology, vol.42, pp.1345-51, 2009.

L. Péret-almeida, A. Cherubino, R. J. Alves, L. Dufossé, and M. Glória, Separation and determination of the physico-chemical characteristics of curcumin, demethoxycurcumin and bisdemethoxycurcumin, Food Res Int, vol.38, issue.8-9, pp.1039-1083, 2005.

I. Chattopadhyay, K. Biswas, U. Bandyopadhyay, and R. K. Banerjee, Turmeric and curcumin: Biological actions and medicinal applications, Current Science, vol.87, issue.1, pp.44-53, 2004.

H. Hayakawa, Y. Minaniya, K. Ito, Y. Yamamoto, and T. Fukuda, Difference of Curcumin Content in Curcuma longa L. (Zingiberaceae) Caused by Hybridization with Other Curcuma Species, Am J Plant Sci, vol.02, issue.02, pp.111-199, 2011.

. Vélo-en, Cossettes de curcuma la mère, 2016.

, Food and Agriculture Organization of the United Nations, 2004.

P. Suresh, H. Manjunatha, and K. Srinivasan, Effect of heat processing of spices on the concentrations of their bioactive principles: Turmeric (Curcuma longa), red pepper (Capsicum annuum) and black pepper (Piper nigrum). J Food Compos Anal, vol.20, pp.346-51, 2007.

K. Srinivasan, Spices as influencers of body metabolism: an overview of three decades of research, Food Res Int, vol.38, issue.1, pp.77-86, 2005.

K. U. Pradeep and P. Geervani, Influence of spices on protein utilisation of winged bean (Prophocarpus tetragonolobus) and horsegram (Dolichos biflorus). Plant Foods Hum Nutr, vol.46, pp.187-93, 1994.

M. A. Azuine and S. V. Bhide, Protective single/combined treatment with betel leaf and turmeric against methyl (acetoxymethyl) nitrosamine-induced hamster oral carcinogenesis, Int J Cancer, vol.51, issue.3, pp.412-417, 1992.

R. Aruna, P. Sathiyarajeswaran, K. Gopakumar, and R. S. Ramaswamy, Cardioprotective effects of kitchen culinaries mentioned in Siddha literature, J Pharmacognosy and Phytochemistry, vol.3, issue.3, pp.71-79, 2004.

J. A. Duke and J. A. Duke, Handbook of medicinal herbs, p.740, 2002.

S. Yodkeeree, W. Chaiwangyen, S. Garbisa, P. Limtrakul, and . Curcumin, demethoxycurcumin and bisdemethoxycurcumin differentially inhibit cancer cell invasion through the down-regulation of MMPs and uPA, J Nutr Biochem, vol.20, issue.2, pp.87-95, 2009.

O. N. Gordon, P. B. Luis, R. E. Ashley, N. Osheroff, and C. Schneider, Oxidative Transformation of Demethoxy-and Bisdemethoxycurcumin: Products, Mechanism of Formation, and Poisoning of Human Topoisomerase II?, Chem Res Toxicol, vol.28, issue.5, pp.989-96, 2015.

Y. N. Teng, Y. W. Hsieh, C. C. Hung, and H. Y. Lin, Demethoxycurcumin Modulates Human P-Glycoprotein Function via Uncompetitive Inhibition of ATPase Hydrolysis Activity, J Agric Food Chem, vol.63, issue.3, pp.847-55, 2015.

G. K. Jayaprakasha, J. Rao, L. Sakariah, and K. K. , Antioxidant activities of curcumin, demethoxycurcumin and bisdemethoxycurcumin, Food Chem. Elsevier, vol.98, issue.4, pp.720-724, 2006.

A. Vandebroek and F. Bauvet, Fondation contre le Cancer. Fonds Anticancer. Compléments alimentaires, curcuma

R. Abou-elkhair, H. A. Ahmed, and S. Selim, Effects of black pepper (piper ,igrum), turmeric powder (curcuma longa) and coriander seeds (coriandrum sativum) and their combinations as feed additives on growth performance, carcass traits, some blood parameters and humoral Immune, Asian-Australas J Anim Sci, vol.27, issue.6, pp.847-54, 2014.

N. G. Das, S. Dhiman, P. K. Talukdar, B. Rabha, D. Goswami et al., Synergistic mosquito-repellent activity of Curcuma longa, Pogostemon heyneanus and Zanthoxylum limonella essential oils, J Infect Public Health, vol.8, issue.4, pp.323-331, 2015.

O. D. Dhingra, G. N. Jham, R. C. Barcelos, F. A. Mendonça, and I. Ghiviriga, Isolation and Identification of the Principal Fungitoxic Component of Turmeric Essential Oil, J Essent Oil Res, vol.19, issue.4, pp.387-91, 2007.

Y. Y. Zhou, H. X. Liu, N. Jiang, X. Y. Feng, X. Y. Feng et al., Elemene, the essential oil of Curcuma wenyujin, inhibits osteogenic differentiation in ankylosing spondylitis, Joint Bone Spine, vol.82, issue.2, pp.100-103, 2015.

V. B. Liju, K. Jeena, and R. Kuttan, Acute and subchronic toxicity as well as mutagenic evaluation of essential oil from turmeric (Curcuma longa L), Food Chem Toxicol, vol.53, pp.52-61, 2013.

S. Ganiger, H. N. Malleshappa, H. Krishnappa, G. Rajashekhar, R. Rao et al., A two generation reproductive toxicity study with curcumin, turmeric yellow, in Wistar rats, Food Chem Toxicol, vol.45, issue.1, pp.64-73, 2007.

J. P. Jourdan, Curcuma et curcumine : de l'histoire aux intérêts thérapeutiques, 2016.

E. Schraufstätter and H. Bernt, Antibacterial action of curcumin and related compounds, Nature, vol.164, issue.4167, pp.456-463, 194910.

J. Schröder, A family of plant-specific polyketide synthases : facts and predictions, Trends Plant Sci, vol.2, issue.10, pp.373-378, 1997.

T. Kita, S. Imai, H. Sawada, H. Kumagai, and H. Seto, The Biosynthetic Pathway of Curcuminoid in Turmeric (Curcuma longa) as Revealed by 13C-Labeled Precursors, Biosci Biotechnol Biochem, vol.72, issue.7, pp.1789-98, 2008.

, Refined exposure assessment for curcumin (E 100), European Food Safety Authority (EFSA), vol.12, issue.10, p.3876, 2014.

. Wiley-online-library, Garrison M, Dayan N. Formulating Cosmetics with Natural Oils, Fats, Butters, and Waxes, 2011.

S. Revathy, S. Elumalai, M. Benny, and A. B. , Isolation, Purification and Identification of Curcuminoids from Turmeric (Curcuma longa L.) by Column Chromatography, J Experimental Sciences, vol.2, issue.7, pp.21-255, 2011.

M. Takenaka, T. Ohkubo, H. Okadome, I. Sotome, T. Itoh et al., Effective Extraction of Curcuminoids by Grinding Turmeric (Curcuma longa) with Medium-chain Triacylglycerols, Food Sci Technol Res, vol.19, issue.4, pp.655-659, 2013.

M. A. Tomren, M. Másson, T. Loftsson, and H. H. Tønnesen, Studies on curcumin and curcuminoids, Int J Pharm, vol.338, issue.1-2, pp.27-34, 2007.

M. Heger, R. F. Van-golen, M. Broekgaarden, and M. C. Michel, The molecular basis for the pharmacokinetics and pharmacodynamics of curcumin and its metabolites in relation to cancer, Pharmacol Rev, vol.66, issue.1, pp.222-307, 2013.

A. P. Gupta, M. M. Gupta, and S. Kumar, Simultaneous determination of curcuminoids in curcuma samples using high performance thin layer chromatography, J Liq Chromatogr Relat Technol, vol.22, issue.10, pp.1561-1570, 1999.

Y. Wang, M. H. Pan, A. L. Cheng, L. I. Lin, Y. S. Ho et al., Stability of curcumin in buffer solutions and characterization of its degradation products, J Pharm Biomed Anal, vol.15, issue.12, pp.1867-76, 1997.

O. N. Gordon, P. B. Luis, H. O. Sintim, and C. Schneider, Unraveling curcumin degradation: autoxidation proceeds through spiroepoxide and vinylether intermediates en route to the main bicyclopentadione, J Biol Chem, vol.290, issue.8, pp.4817-4845, 2015.

A. Khurana and C. T. Ho, High Performance Liquid Chromatographic Analysis of Curcuminoids and Their Photo-oxidative Decomposition Compounds in Curcuma Longa L, J Liq Chromatogr, vol.11, issue.11, pp.2295-304, 1988.

A. Kunwar, A. Barik, R. Pandey, and K. I. Priyadarsini, Transport of liposomal and albumin loaded curcumin to living cells: An absorption and fluorescence spectroscopic study, Biochim Biophys Acta, issue.10, pp.1513-1533, 1760.

R. K. Gangwar, G. B. Tomar, V. A. Dhumale, S. Zinjarde, R. B. Sharma et al., Curcumin conjugated silica nanoparticles for improving bioavailability and its anticancer applications, J Agric Food Chem, vol.61, issue.40, pp.9632-9639, 2009.

H. Yan, C. Teh, S. Sreejith, L. Zhu, A. Kwok et al., Functional mesoporous silica nanoparticles for photothermal-controlled drug delivery in vivo, Angew Chem Int Ed Engl, vol.51, issue.33, pp.8373-8380, 2012.

R. K. Gangwar, V. A. Dhumale, D. Kumari, U. T. Nakate, S. W. Gosavi et al., Conjugation of curcumin with PVP capped gold nanoparticles for improving bioavailability, Mater Sci Eng C. Elsevier, vol.32, issue.8, pp.2659-63, 2012.

S. Hatamie, M. Nouri, S. K. Karandikar, A. Kulkarni, S. D. Dhole et al., Complexes of cobalt nanoparticles and polyfunctional curcumin as antimicrobial agents, Mater Sci Eng C. Elsevier, vol.32, issue.2, pp.92-99, 2012.

S. Ravindra, A. F. Mulaba-bafubiandi, V. Rajinikanth, K. Varaprasad, N. Reddy et al., Development and Characterization of Curcumin Loaded Silver Nanoparticle Hydrogels for Antibacterial and Drug Delivery Applications, J Inorg Organomet Polym Mater, vol.22, issue.6, pp.1254-62, 2012.

M. Yallapu, S. F. Othman, E. T. Curtis, N. A. Bauer, N. Chauhan et al., Curcuminloaded magnetic nanoparticles for breast cancer therapeutics and imaging applications, Int J Nanomedicine, vol.7, pp.1761-79, 2012.

M. M. Yallapu, M. C. Ebeling, S. Khan, V. Sundram, N. Chauhan et al., Novel curcumin-loaded magnetic nanoparticles for pancreatic treatment, Mol Cancer Ther, vol.12, issue.8, pp.1471-80, 2013.

T. M. Kolev, E. A. Velcheva, B. A. Stamboliyska, and M. Spiteller, DFT and experimental studies of the structure and vibrational spectra of curcumin, Int J Quantum Chem, vol.102, issue.6, pp.1069-79, 2005.

E. M. Williamson, Major herbs of ayurveda, Churchill Livingstone, vol.361, p.276, 2002.

S. Gao, G. Xia, L. Wang, L. Zhou, F. Zhao et al., Sesquiterpenes from Curcuma wenyujin with their inhibitory activities on nitric oxide production in RAW 264.7 cells, Nat Prod Res, vol.31, issue.5, pp.548-54, 2017.

V. B. Liju, K. Jeena, and R. Kuttan, Chemopreventive activity of turmeric essential oil and possible mechanisms of action, Asian Pac J Cancer Prev, vol.15, issue.16, pp.6575-80, 2014.

G. C. Armitage, Development of a Classification system for periodontal diseases and conditions, Ann Periodontol, vol.4, issue.1, pp.1-6, 1999.

J. G. Caton, G. Armitage, T. Berglundh, I. Chapple, S. Jepsen et al., A new classification scheme for periodontal and peri-implant diseases and conditions -Introduction and key changes from the 1999 classification, J Clin Periodontol, vol.45, pp.1-8, 2018.

C. Mattout, B. Houvenaeghel, G. Rachlin, and P. Mattout, Nouvelle classification des conditions saines et pathologiques des tissus parodontaux et péri-implantaires, Journal de Parodontologie & d'Implantologie Orale, vol.37, pp.1-10, 2018.

N. P. Lang and P. M. Bartold, Periodontal health, J Clin Periodontol, vol.45, pp.9-16, 2018.

I. Chapple, Periodontal health and Gingivitis. European Federation of Periodontology, 2019.

S. Murakami, B. L. Mealey, A. Mariotti, and I. Chapple, Dental plaque-induced gingival conditions, J Clin Periodontol, vol.45, pp.17-27, 2018.

P. Holmstrup, J. Plemons, and J. Meyle, Non-plaque-induced gingival diseases, J Clin Periodontol, vol.45, pp.28-43, 2018.

P. N. Papapanou, M. Sanz, N. Buduneli, T. Dietrich, M. Feres et al., Periodontitis: Consensus report of workgroup 2 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions: Classification and case definitions for periodontitis, J Clin Periodontol, vol.89, issue.1, pp.173-182, 2018.

M. S. Tonetti, H. Greenwell, and K. S. Kornman, Staging and grading of periodontitis: Framework and proposal of a new classification and case definition, J Periodontol, vol.89, pp.159-72, 2018.

H. Chardin, O. Barsotti, and M. Bonnaure-mallet, Microbiologie en odonto-stomatologie, 2006.

S. S. Socransky and A. D. Haffajee, Dental biofilms: difficult therapeutic targets, Periodontol, vol.28, pp.12-55, 2000.

P. Marsh and M. Martin, Oral Microbiology. 5th ed. Edinburgh, p.222, 2009.

P. D. Marsh, Dental plaque as a biofilm and a microbial community -implications for health and disease, BMC Oral Health, vol.6, issue.S1, p.14, 2006.

P. Marsh, Plaque as a biofilm: pharmacological principles of drug delivery and action in the sub-and supragingival environment, Oral Dis, vol.9, issue.s1, pp.16-22, 2003.

R. J. Lamont and H. F. Jenkinson, Oral microbiology at a glance, vol.85, 2010.

H. F. Jenkinson and R. J. Lamont, Oral microbial communities in sickness and in health, Trends Microbiol, vol.13, issue.12, pp.589-95, 2005.

J. Li, E. J. Helmerhorst, C. W. Leone, R. F. Troxler, T. Yaskell et al., Identification of early microbial colonizers in human dental biofilm, J Appl Microbiol. déc, vol.97, issue.6, pp.1311-1319, 2004.

B. Nyvad and M. Kilian, Microbiology of the early colonization of human enamel and root surfaces in vivo, Eur J Oral Sci, vol.95, issue.5, pp.369-80, 1987.

F. Simain, E. Rompen, and E. Heinen, Biofilms bactériens et médecine dentaire, Rev Med Liège, vol.65, issue.10, pp.569-573, 2010.

D. J. Bradshaw, P. D. Marsh, G. K. Watson, and C. Allison, Role of Fusobacterium nucleatum and coaggregation in anaerobe survival in planktonic and biofilm oral microbial communities during aeration, Infect Immun, vol.66, issue.10, pp.4729-4761, 1998.

F. Mikx and J. S. Van-der-hoeven, Symbiosis of Streptococcus mutans and Veillonella alcalescens in mixed continuous cultures, Arch Oral Biol, vol.20, issue.7, pp.407-417, 1975.

B. C. Mcbride and J. S. Van-der-hoeven, Role of interbacterial adherence in colonization of the oral cavities of gnotobiotic rats infected with Streptococcus mutans and Veillonella alcalescens, Infect Immun, vol.33, issue.2, pp.467-72, 1981.

P. R. Murray and E. J. Baron, Manual of clinical microbiology, 2007.

G. Hajishengallis, R. P. Darveau, and M. A. Curtis, The keystone-pathogen hypothesis, Nat Rev Microbiol, vol.10, issue.10, pp.717-742, 2012.

H. K. Datta, W. F. Ng, J. A. Walker, S. P. Tuck, and S. S. Varanasi, The cell biology of bone metabolism, J Clin Pathol, vol.61, issue.5, pp.577-87, 2008.

N. Bostanci and G. N. Belibasakis, Porphyromonas gingivalis: an invasive and evasive opportunistic oral pathogen, FEMS Microbiol Lett, vol.333, issue.1, pp.1-9, 2012.

C. Bodet, F. Chandad, and D. Grenier, Potentiel pathogénique de Porphyromonas gingivalis, Treponema denticola et Tannerella forsythia, le complexe bactérien rouge associé à la parodontite, Pathol Biol, vol.55, issue.3-4, pp.154-62, 2007.

K. Y. How, K. P. Song, and K. G. Chan, Porphyromonas gingivalis: An Overview of Periodontopathic Pathogen below the Gum Line. Front Microbiol, 2016.

,

A. C. Tanner and J. Izard, Tannerella forsythia, a periodontal pathogen entering the genomic era, Periodontol, vol.42, pp.88-113, 2000.

D. Haubek, O. K. Ennibi, K. Poulsen, S. Poulsen, N. Benzarti et al., Early-onset periodontitis in Morocco is associated with the highly leukotoxic clone of Actinobacillus actinomycetemcomitans, J Dent Res, vol.80, issue.6, pp.1580-1583, 2001.

D. H. Fine, A. G. Patil, and S. K. Velusamy, Aggregatibacter actinomycetemcomitans (Aa) Under the Radar: Myths and Misunderstandings of Aa and Its Role in Aggressive Periodontitis. Front Immunol, vol.10, p.728, 2019.

E. J. Leys, A. L. Griffen, S. J. Strong, and P. A. Fuerst, Detection and strain identification of Actinobacillus actinomycetemcomitans by nested PCR, J Clin Microbiol, vol.32, issue.5, pp.1288-94, 1994.

S. Izui, S. Sekine, K. Maeda, M. Kuboniwa, A. Takada et al., Antibacterial Activity of Curcumin Against Periodontopathic Bacteria. J Periodontology, vol.87, issue.1, pp.1-18, 2015.

A. M. Sha and B. T. Garib, Antibacterial Effect of Curcumin against Clinically Isolated Porphyromonas gingivalis and Connective Tissue Reactions to Curcumin Gel in the Subcutaneous Tissue of Rats, Biomed Res Int, p.6810936, 2019.

G. Chemical, Page consultée le 20/11/2019). DMSO Physical Properties

Z. Mahdi, G. Habiboallh, N. N. Mahbobeh, Z. J. Mina, Z. Majid et al., Lethal effect of blue light-activated hydrogen peroxide, curcumin and erythrosine as potential oral photosensitizers on the viability of Porphyromonas gingivalis and Fusobacterium nucleatum. Laser Ther, vol.24, pp.103-114, 2015.

F. Keihanian, A. Saeidinia, R. K. Bagheri, T. P. Johnston, and A. Sahebkar, Curcumin, hemostasis, thrombosis, and coagulation, J Cell Physiol, vol.233, issue.6, pp.4497-4511, 2018.

, Annexe 4 : Composition des géloses Colombia au sang

. Pour-1l-d'eau-distillée,

, Annexe 5 : Protocole de la coloration de Gram

, Nettoyer une lame de verre à l'alcool et y déposer une goutte d'H2O stérile

, Prélever une colonie à l'aide d'une pipette Pasteur préalablement passée dans la flamme d'un bec Bunsen et les mettre en suspension dans la goutte d

, Sécher la lame sur une plaque chauffante

, Fixer l'échantillon en passant la lame 3 fois dans la flamme d'un bec Bunsen

, Déposer quelques gouttes de solution de cristal violet et laisser agir 1 min

, Eliminer l'excès de colorant

, Déposer quelques gouttes de lugol et laisser agir 1 minute

, Eliminer l'excès de colorant

, Décolorer rapidement à l'alcool

, Déposer quelques gouttes de safranine et laisser agir 1 minute

, Eliminer l'excès de colorant

, Sécher la lame sur une plaque chauffante

, Déposer une goutte d'huile à immersion et observer au microscope optique avec l'objectif à immersion 100

L. Vu, . Président, and . Jury,

L. Vu and . Directrice,