W. P. Rowe, R. J. Huebner, L. K. Gilmore, R. H. Parrott, and T. G. Ward, Isolation of a cytopathogenic agent from human adenoids undergoing spontaneous degeneration in tissue culture, Proc. Soc. Exp. Biol. Med, vol.84, issue.3, pp.570-573, 1953.

G. Smith, C. Wiethoff, and L. Phoebe, Adenovirus, Curr Top Microbiol Immunol, vol.343, pp.195-224, 2010.
URL : https://hal.archives-ouvertes.fr/hal-00426344

T. Lion, Adenovirus infections in immunocompetent and immunocompromised patients, Clin Microbiol Reviews, vol.27, issue.3, pp.441-62, 2014.

G. Newerow and J. Flint, Lessons learned from Adenovirus, vol.593, pp.3395-3418, 1970.

L. Tiantian and Z. Zhichao, Xingui T « A recombinant trivalent vaccine candidate against human adenovirus types 3, 7, and 55, Vaccine, vol.36, pp.2199-2206, 2018.


A. A. Macmahon and E. , Adenovirus Infection, Viral Infections, vol.45, pp.777-780, 2017.

M. Chen and Z. Zhu, Adenoviruses associated with acute respiratory diseases reported in Beijing from 2011 to, PloS ONE, vol.10, issue.3, p.121375, 2013.

S. Pereira and C. Florêncio, Adenoviruses and acute respiratory infections in children living in an equatorial area of Brazil, Epidemiol. Infect, vol.144, pp.355-362, 2016.

S. Matthes-martin and T. Feuchtinger, European guidelines for diagnosis and treatment of adenovirus infection in leukemia and stem cell transplantation, Transplant Infectious Disease, vol.14, pp.555-63, 2012.

J. Brouard and A. Vabret, « Prise en charge des pathologies respiratoires à Adénovirus chez l'enfant immunocompétent, Antibiotiques, vol.6, pp.97-102, 2004.

C. J. Barnadas and D. Schimdt, Molecular epidemiology of human adenovirus infections in Denmark, Journal of Clinical Virology, vol.104, pp.16-22, 2018.

J. Hong and H. Lee, Lower Respiratory Tract Infections due to Adenovirus in Hospitalized Korean Children : Epidemiology, Clinical Features, and Prognosis

, Clinical Infectious Diseases, vol.32, pp.1423-1432, 2001.

R. C. Singh and G. , Computational analysis and identification of an emergent human adenovirus pathogen implicated in a respiratory fatality, Virology, 2011.

, , vol.409, pp.121-147

G. Viquesnel and A. Vabret, Pneumopathie sévère à Adenovirus type 7 chez un adulte imunocompétent, Ann Fr Anesth Réanim, vol.16, pp.50-52, 1997.

A. Amold and E. Macmahon, Adenovirus Infections" Viral Infection, 2017.

T. Bourcier and B. Bodaghi, Les infections oculaires, Ophtalmologie de France, 2010.

M. Echavarria, « Adenoviruses in Immunocompromised hosts, Clinical Microbiology Reviews, vol.21, pp.704-715, 2008.

N. Nikitenko and T. Speiseder, Targeting species D adenoviruses replication to counteract the epidemic keratoconjunctivitis, Biochimie, vol.113, pp.10-16, 2015.


P. Kinchington and E. Romanowski, Prospect for adenovirus antivirals, Journal of Antimicrobial Chemotherapy, vol.55, pp.424-429, 2005.

A. Combes and C. Luyt, Myocardites aiguës ". Reanimation, vol.14, pp.248-254, 2005.

E. Ponterio and L. Gnessi, Adénovirus 36 and obesity: an overview, Viruses, 2015.

S. Esposito and V. Preti, Adenovirus 36 infection and obesity, Journal of Clinical Virology, vol.55, pp.95-100, 2012.

C. Gabbert and M. Donohue, Adenovirus 36 and obesity in children and adolescents, Pediatrics, vol.126, pp.721-726, 2010.

M. Leruez-ville, Diagnostic virologique des infections respiratoires ». Archives de Pédiatrie, vol.14, pp.404-409, 2007.

F. Freymuth and A. Vabret, Techniques actuelles de diagnostic des infections virales respiratoires en réanimation, vol.16, pp.200-209, 2007.

C. Bretonnière and C. Touzeau, Défaillance multiviscérale et infection disséminée à adénovirus, Médecine et maladies infectieuses, pp.296-298, 2010.

J. Zahradnik and J. Mary, Adenovirus infection in the immunocompromised patient

, The American journal of Medicine, vol.68, pp.725-732, 1980.

J. Putra and A. Suriawinata, Adenovirus hepatitis presenting as a tumoral lesions in an immunocompromised patient, Annals in Hepatology, vol.13, pp.827-829, 2014.

B. A. Ronan, Fulminant hepatitis due to human adenovirus, Infection, vol.42, pp.105-111, 2014.

K. Nanmoku and N. Ishikawa, Clinical characteristics and outcomes of adenovirus infection of the urinary tract after renal transplantation, Transplant Infectious Disease, vol.18, pp.234-239, 2016.

T. Lion and K. Kosulin, Monitoring of adenovirus load in stool by real-time PCR permits early detection of impending invasive infection in patients after allogenic stem cell transplantation, Leukemia, vol.24, pp.706-714, 2010.

A. Heim and C. Ebnet, Rapid and quantitative detection of human adenovirus DNA by real-time PCR, Journal of Medical Virology, vol.70, pp.228-239, 2003.

S. Jiang and H. Dezfulian, Real-time quantitative PCR for enteric adenovirus serotype 40 in environmental waters, Can. J. Microbiol, vol.51, pp.393-398, 2005.

Z. Gu, W. Belzer, and . Multiplexed, Real-time PCR for quantitative detection of human adenovirus, Journal of Clinical Microbiology, vol.41, pp.4636-4641, 2003.

B. Marchou and G. Martin-blondel, Conduite à tenir devant une diarrhée infectieuse

E. Pilly, , pp.153-157, 2018.

F. Legrand and D. Berrebi, Early diagnosis of adenovirus infection and treatment with cidofovir after bone marrow transplantation in children, Bone Marrow Transplantation, vol.27, pp.621-626, 2001.

K. Taniguchi and S. Yoshihara, Incidence and treatment strategy for disseminated adenovirus disease after haploidentical stem cell transplantation, Ann Hematology, vol.91, pp.1305-1312, 2012.

D. F. Florescu and . Pergam, Safety and efficacy of CMX001 as salvage therapy for severe adenovirus infections in immunocompromised patients, Biol Blood Marrow Transplant, vol.18, pp.731-738, 2012.

W. Wold and K. Toth, New drug on the horizon for treating adenovirus, Expert Opinion on Pharmacotherapy, vol.16, pp.2095-2099, 2015.

P. Hiwarkar and P. Amrolia, Brincidofovir is highly efficacious in controlling adenoviromia in pediatric recipients oh hematopoietic cell transplant, Blood, 2017.

, , vol.129, pp.2033-2037

T. Van-genechten and J. Van-heerden, Successful treatment of Adenovirus infection with Brincidofovir in an Immunocompromised patient after hematological stem cell transplantation, Case Reports in Infectious Diseases, 2020.

P. Bordigoni and A. S. Carret, Treatment of Adenovirus Infections in Patients Undergoing Allogeneic Hematopoietic Stem Cell Transplantation, Clinical Infectious Diseases, vol.32, pp.1290-1297, 2001.

Y. Ho, Q. Suai-tan, and C. , The use of intravesical cidofovir for the treatment of adenovirus-associated heamorrhagic cystitis in a kidney transplant recipient

, Clinical Kidney Journal, vol.12, pp.745-747, 2019.

P. Bordigoni, A. S. Carret, V. Venard, F. Witz, L. Faou et al., Treatment of adenovirus infections in patients undergoing allogeneic hematopoietic stem cell transplantation, Clin Infect Dis, vol.32, issue.9, pp.1290-1297, 2001.

K. Taniguchi and S. Yoshihara, Incidence and treatment strategy for disseminated adenovirus disease after haploidentical stem cell transplantation, Ann Hematol, vol.91, pp.1305-1312, 2012.

R. Potter and J. Cantrell, Adenovirus-associated Deaths in US Military during Postvaccination Period, Emerging Infectous Desease, vol.18, issue.3, 1999.

M. Mcneil and I. Paradowska-stankiewicz, Adverse events following adenovirus type 4 and type 7 vaccine, live, oral, in the VAERS, 2011.

, Vaccine, vol.37, pp.6760-6767, 2019.

G. Gray and P. Gosxami, Adult Adenovirus Infectious: Loss of Orphaned Vaccines Precipitates Military Respiratory Disease Epidemics, Clinical Infectious Diseases, vol.31, pp.663-70, 2000.

R. Miura-ochiai and Y. Shimada, «Quantitative Detection and Rapid Identification of Human Adenoviruses, Journal of Clinical Microbiology, pp.958-967, 2007.

, Roche Applied Science, LightCycler 480 Probes Master, 2005.

Y. Yasumura and Y. Kawakita, Studies on SV40 in tissue culture -preliminary step for cancer research "in vitro, Nihon Rinshon, vol.21, pp.1201-1215, 1963.

M. Bourtros and F. Heigwer, Microscopy-Based High-Content Screening, vol.163, pp.1314-1325, 2015.

C. Vivek and D. Abraham, High content screening applied to large-scale cell biology

, Biotechnology, vol.22, pp.15-22, 2004.

M. Mattiazzi, U. Erin, and B. , High-Content Screening for quantitative cell biology, Trends in Cell Biology, vol.26, pp.58-611, 2016.

P. Brodin and T. Christophe, High-content screening in infectious diseases, Current Opinion in Chemical Biology, vol.15, pp.534-539, 2011.

C. Scheeder and F. Heigwer, Machine learning and image-based profiling in drug discovery, Current Opinion in Systems Biology, vol.10, pp.43-52, 2018.

K. Hickman and S. Hariharan, Image-based analysis of protein stability, Cytometry, vol.97, pp.363-377, 2020.

W. Y. Winnie and Q. Waseem, Management of Adenovirus in Children after Allogeneic Hematopoietic Stem Cell Transplantation, Advances in Hematology, pp.1-12, 2013.


. Références,

V. C. Abraham, D. L. Taylor, and J. R. Haskins, High content screening applied to large-scale cell biology, Trends in Biotechnology, vol.22, issue.1, pp.15-22, 2004.

D. Thouvenot, G. Billaud, and F. Morfin, Actualité de la culture cellulaire et de son application au diagnostic des infections virales, Virologie, vol.8, issue.4, pp.297-309, 2004.

R. Francis, Y. Ominami, B. Khalil, J. Y. , L. Scola et al., High-throughput isolation of giant viruses using high-content screening, Communications biology, vol.2, p.216, 2019.
URL : https://hal.archives-ouvertes.fr/hal-02445597

, Conclusion et Perspectives Introduction Les adénovirus sont des virus à ADN bicaténaire linéaire, non enveloppés, à capside icosaédrique, appartenant à la famille des Adenoviridae. Ils sont responsables d'un grand nombre d'infections (gastroentérites, conjonctivites, etc), dont certaines pouvant être sévères chez la personne immunodéprimée. Le diagnostic actuel se fait par PCR, la culture virale reste un outil clé pour la détection et la caractérisation des différentes souches

, Récemment nous avons développé une nouvelle méthode d'isolement et de détection des virus basée sur la culture virale et couplée à la microscopie haut débit

, La microscopie High Content Screening (HCS) consiste en l'acquisition d'images par le microscope, puis l'analyse des datas générées par l'appareil et le développement d'algorithmes permettant l'analyse des échantillons à grande échelle. L'objectif de ce travail a été la mise au point de cette nouvelle technique de détection automatisée de la réplication en culture