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, Comité de l'antibiogramme de la Société Française de Microbiologierecommandations

P. Tomlin, C. Sand, and R. P. Rennie, Evaluation of E Test, disk diffusion and broth microdilution to establish tentative quality control limits and review susceptibility breakpoints for two aerobic actinomycetes, Diagn Microbiol Infect Dis. 1 août, vol.40, issue.4, pp.179-86, 2001.

W. Lowman and N. Aithma, Antimicrobial susceptibility testing and profiling of Nocardia species and other aerobic actinomycetes from South Africa: comparative evaluation of broth microdilution versus the Etest, J Clin Microbiol, vol.48, issue.12, pp.4534-4574, 2010.

L. Huang, X. Chen, H. Xu, L. Sun, C. Li et al., Clinical features, identification, antimicrobial resistance patterns of Nocardia species in China, Diagn Microbiol Infect Dis. juin, vol.94, issue.2, pp.165-72, 2009.

A. Gomez-flores, O. Welsh, S. Said-fernández, G. Lozano-garza, R. Tavarez-alejandro et al., vitro and in vivo activities of antimicrobials against Nocardia brasiliensis, vol.48, pp.832-839, 2004.

J. R. Biehle, S. J. Cavalieri, M. A. Saubolle, and L. J. Getsinger, Comparative evaluation of the E test for susceptibility testing of Nocardia species, Diagn Microbiol Infect Dis. 1 juin, vol.19, issue.2, pp.101-111, 1994.

D. Perçin, B. Sümerkan, and I. R. , Comparative evaluation of e-test and disk diffusion methods for susceptibility testing of Nocardia species

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A. Ambaye, P. C. Kohner, P. C. Wollan, K. L. Roberts, G. D. Roberts et al., Comparison of agar dilution, broth microdilution, disk diffusion, E-test, and BACTEC radiometric methods for antimicrobial susceptibility testing of clinical isolates of the Nocardia asteroides complex, J Clin Microbiol. avr, vol.35, issue.4, pp.847-52, 1997.

B. A. Brown-elliott, J. Killingley, S. Vasireddy, L. Bridge, and R. J. Wallace, In Vitro Comparison of Ertapenem, Meropenem, and Imipenem against Isolates of Rapidly Growing Mycobacteria and Nocardia by Use of Broth Microdilution and Etest, J Clin Microbiol. juin, vol.54, issue.6, pp.1586-92, 2016.

D. Haussaire, P. Fournier, K. Djiguiba, V. Moal, T. Legris et al., Nocardiosis in the south of France over a 10-years period, Int J Infect Dis IJID Off Publ Int Soc Infect Dis. avr, vol.57, pp.13-20, 2004.
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D. Lebeaux, R. Freund, C. Van-delden, H. Guillot, S. D. Marbus et al., Outcome and Treatment of Nocardiosis After Solid Organ Transplantation: New Insights From a European Study, Clin Infect Dis. 15 mai, vol.64, issue.10, pp.1396-405, 2017.
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A. Galacho-harriero, P. D. Delgado-lópez, M. P. Ortega-lafont, J. Martín-alonso, J. M. Castilla-díez et al., Nocardia farcinica Brain Abscess: Report of 3 Cases. World Neurosurg, vol.106, pp.1053-1068, 2017.

A. Gieger, S. Waller, and J. Pasternak, Nocardia Arthritidis Keratitis: Case Report and Review of the Literature, Nepal J Ophthalmol Biannu Peer-Rev Acad J Nepal Ophthalmic Soc NEPJOPH, vol.9, issue.18, pp.91-95, 2017.

N. Sharma and S. O'hagan, The role of oral co-trimoxazole in treating Nocardia farcinica keratitis-a case report, J Ophthalmic Inflamm Infect. déc, vol.6, issue.1, p.23, 2016.

A. Verner, A. Durrani, R. P. Kowalski, and V. Jhanji, A Case of Nocardia farcinica Keratitis in a Pediatric Contact Lens Wearer, Eye Contact Lens. mars, vol.46, issue.2, pp.11-13, 2020.

R. B. Rush, Contact lens-associated nocardial necrotizing scleritis, Korean J Ophthalmol KJO. août, vol.27, issue.4, pp.291-294, 2013.

, Transférer le matériel biologique (une à plusieurs colonies) à l'aide d'une oëse stérile

, Mélanger jusqu'à obtention d'une solution trouble

, Ajouter 900µL d'éthanol absolu

, Retirer la totalité du surnageant à l'aide d'une pipette

, Laisser sécher en air ambiant le culot pendant 5 minutes

, Ajouter 25µL d'acide formique (70%) et mélanger à l'aide d'une pipette jusqu'à ce que le culot soit dissout

, A jouter 25µL d'acétonitrile (100%) en mélangeant le tout 2 à 3 fois par aspiration-refoulement

, Déposer 1µL du surnageant sur la cible MALDI

L. Sécher,

, Spectres de Nocardia, base de données MALDI-TOF (IHU, AP-HM) Nombre de spectres, vol.2, p.129

, Nombre d'espèces différentes, p.54