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, Transférer le matériel biologique (une à plusieurs colonies) à l'aide d'une oëse stérile
, Mélanger jusqu'à obtention d'une solution trouble
, Ajouter 900µL d'éthanol absolu
, Retirer la totalité du surnageant à l'aide d'une pipette
, Laisser sécher en air ambiant le culot pendant 5 minutes
, Ajouter 25µL d'acide formique (70%) et mélanger à l'aide d'une pipette jusqu'à ce que le culot soit dissout
, A jouter 25µL d'acétonitrile (100%) en mélangeant le tout 2 à 3 fois par aspiration-refoulement
, Déposer 1µL du surnageant sur la cible MALDI
,
, Spectres de Nocardia, base de données MALDI-TOF (IHU, AP-HM) Nombre de spectres, vol.2, p.129
, Nombre d'espèces différentes, p.54