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Master Thesis

Caractérisation de QTL contrôlant des traits racinaires chez le Mil

Abstract : Pearl millet, Pennisetum glaucum, is the staple food of more than 90 million people in sub-Saharan Africa and India, and global millet production is limited by the drought and low soil fertility that characterize its growing area. Improving its root system could help optimize the acquisition of water and mineral elements. Knowledge of the genetic basis that controls the establishment of this root system may allow the use of marker-assisted selection, a powerful tool that fixes the selection on the selected traits. The study of the root architecture and root growth dynamics of millet has made it possible to highlight certain root traits of interest: early growth of the seminal root and rhizospheric soil aggregation. Preliminary studies led to the detection of several QTLs controlling these traits by association genetics. The objective of this training course was to validate these QTLs using two types of population coming from crosses between two contrasted parents. We first tested 4 methods for QTL detection using bulk segregant analysis. Simulations were conducted to evaluate the impact of the QTL effect and number of individuals on QTL detection accuracy on these methods. Beside, we initiated the characterization of a population of backcross inbred lines (BC2F4). We evaluated the homogeneity of a sample of BC2F4 lines using genotypic and phenotypic methods. We observed inter-linear heterogeneity and intra-linear homogeneity. This work will help detect QTLs of interest.
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Submitted on : Tuesday, December 1, 2020 - 10:25:53 AM
Last modification on : Saturday, July 16, 2022 - 3:30:21 AM
Long-term archiving on: : Tuesday, March 2, 2021 - 6:37:52 PM


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  • HAL Id : dumas-03032947, version 1


Princia Aurore Biawallys Nakombo-Gbassault. Caractérisation de QTL contrôlant des traits racinaires chez le Mil. Agronomie. 2020. ⟨dumas-03032947⟩



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