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Master Thesis

Revue de la littérature sur la région non codante du BK Virus : illustration par l'étude de la région non codante du BK Virus chez 18 patients bordelais

Abstract : BK Virus (BKpyV) is a DNA virus discovered in 1971 which belongs to the Polyomaviridae family. This virus is restricted to humans and widely spread within the population. After primary infection the virus establishes latency in kidneys and the urinary tract. Primary infection and reactivation are usually asymptomatic but may turn into a severe threat for immunocompromised patients who have undergone renal transplantation or hematopoietic stem cell transplantations. BKPyV non coding control region is remarkable for its plasticity. It can be found in wild-type conformation, called “archetype”, or rearranged conformation which harbors block duplications and/or deletions. Through its interaction with cellular transcription factors, it orchestrates the replication of viral DNA as well as the transcription of early and late viral genes. The source and significance of such rearrangements remain unclear. Experimental data suggest that rearranged BKPyV could be associated with increased pathogenicity, enlarged cellular tropism or oncogenic properties. However, these features need to be confirmed in vivo. In our study, we have sequenced then analyzed the non-coding control region of BKPyV found to replicate at high level in the urine of 18 patients after hematopoietic stem cell transplantation between 04/01/2019 and 03/31/2020 at University hospital of Bordeaux in France. All of the sequenced BKPyV non-coding control regions harbored archetype conformation. Thus, rearrangements of the non-coding control region seem to be rare in this context and not associated with high level replication of the BKPyV and BKPyV hemorrhagic cystitis. Besides, our archetype sequences revealed nucleotide polymorphism. A predictive analysis showed that those variations could affect transcription factor binding sites distribution among the non-coding control region. Whether such variations influence BKPyV gene transcription remains to be clarified in further studies.
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Submitted on : Wednesday, December 9, 2020 - 2:25:24 PM
Last modification on : Tuesday, January 26, 2021 - 3:10:16 AM
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  • HAL Id : dumas-03048592, version 1



Jordi Pacaud. Revue de la littérature sur la région non codante du BK Virus : illustration par l'étude de la région non codante du BK Virus chez 18 patients bordelais. Sciences du Vivant [q-bio]. 2020. ⟨dumas-03048592⟩



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