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Master Thesis

Étude ID-MUT : mise en place de la détermination des mutations de l’EGFR par technique Idylla™ dans le cancer du poumon : évaluation chez 225 patients du CHU de Caen et du Centre de Lutte Contre le Cancer François Baclesse de Caen

Abstract : In advanced non-small-cell lung carcinomas (NSCLC) and non-epidermoid, the first line of treatment should be decided only with knowledge of driver mutations (ROS1, ALK and EGFR) and PD-L1 status. In Caen, the implementation of the Idylla™ EGFR rapid technique was motivated by clinicians in order to speedup their patients care/treatment. The purpose of the prospective ID-MUT study was to develop and evaluate a rapid targeted analysis of the EGFR mutation by Idylla™ technique and compare it to the Next Generation Sequencing (NGS) ion Torrent technique on a cohort of 225 patients followed at Caen. A total of 194 tumors samples from patients with formalin-fixed and paraffin-embedded CBPNPC have been simultaneously tested using both techniques. The comparison focused on the concordance and time to analyze the two techniques. The total agreement was excellent: 98.95% (188/190). Only 6 cases were inconsistent: 2 false negatives and 4 excluded from the analysis due to a non-objectifiable mutation by the Idylla™ technique. The analysis time of the Idylla™ technique was 6.6 working days versus 15.8 working days for the NGS technique (p value <0.001). The easy-to-use Idylla™ technology allows the sensitive and rapid characterization of a majority of EGFR mutations and can be optimized by using the Idylla™ Explore tool exploiting the amplification curves or increasing the amount of initial hardware. However, the use of previously extracted DNA may be judicious in order to optimize the material, as further analysis by NGS remains essential.
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https://dumas.ccsd.cnrs.fr/dumas-03572453
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Submitted on : Monday, February 14, 2022 - 11:44:03 AM
Last modification on : Saturday, April 2, 2022 - 3:38:36 AM
Long-term archiving on: : Sunday, May 15, 2022 - 6:47:02 PM

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  • HAL Id : dumas-03572453, version 1

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Adèle Riot. Étude ID-MUT : mise en place de la détermination des mutations de l’EGFR par technique Idylla™ dans le cancer du poumon : évaluation chez 225 patients du CHU de Caen et du Centre de Lutte Contre le Cancer François Baclesse de Caen. Médecine humaine et pathologie. 2021. ⟨dumas-03572453⟩

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