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Master Thesis

Influence de la conservation du culot globulaire canin sur la composition électrolytique du produit transfusé

Abstract : Red Blood Cell Concentrate is a concentrate of erythrocytes obtained by centrifugation of fresh whole blood. According to actual recommendations, this concentrate can be stored on CPDA anticoagulant at low temperatures for up to 42 days. Nonetheless, previous studies in both human and veterinary medicine tend to show that changes appear in those products long before those recommended 42 days. This prospective study was performed with the Blood Bank of the National Veterinary School of Alfort (ENVA). The aim of the study was to understand and to quantify changes appearing during the storage of packed Red Blood Cell Concentrate. Five Red Blood Cell Concentrates were collected from healthy Golden Retriever dogs and stored at low temperature in CPDA anticoagulant for 42 days. Analysis were performed on the drawing day, referred as J0 in this study, and on days J1, J3 J7, J14, J21, J28, J35 and J42. A complete biochemistry, blood gas, electrolytes and a complete blood count were performed at each defined time. The data collected show a decrease in pH and in glucose and bicarbonates concentration, and an increase in ammonium, lactate, sodium, potassium and chloride concentrations. Haematological parameters showed no significant changes during storage. Those results are similar to studies performed in human medicine. Still, some parameter like potassium seems to show a smaller variation compared to those reported in human medicine. Further studies in the veterinary field are needed to know whether transfusion of long time stored Canine Red Blood Cell Concentrates can have an impact on some specific patient’s survival. Nevertheless, our observations suggest that a 35 to 42 day long storage of Canine Red Blood Cell Concentrates is acceptable.
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Master Thesis
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https://dumas.ccsd.cnrs.fr/dumas-03630119
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Submitted on : Thursday, April 7, 2022 - 11:14:04 AM
Last modification on : Thursday, April 21, 2022 - 3:57:52 PM

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Ariane Willame. Influence de la conservation du culot globulaire canin sur la composition électrolytique du produit transfusé. Sciences du Vivant [q-bio]. 2022. ⟨dumas-03630119⟩

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