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Master Thesis

Évaluation de l’impact de l’humidité résiduelle dans les plateaux opératoires après stérilisation

Abstract : Ambient air and dust are germ carriers. After sterilisation, air passes through the packaging which content remains sterile until the expiration date. When this sterile composition presents traces of moisture, a potential risk of re-contamination is suspected. Re-contamination may occur during storage and until use. Our study aims to assess the impact of the residual moisture in the surgical set after sterilisation. The study was focused on two series of six compositions to compare two types of packaging: container and nonwoven. Each of the six compositions tested contained 15 ceramic carriers fostering the adhesion of microorganisms. After a cycle sterilisation (135 °C-18 minutes) without a drying phase to obtain residual water, the compositions are stored under adverse conditions. Residual water and ceramics were recovered aseptically and cultured at 35 ± 1°C for 14 days. Positive controls, consisting in perforated packaging, were performed in parallel. Each series was repeated 5 times to assess contamination after 0, 1, 3, 7 and 14 days of storage. Over the 210 tubes cultured, and regardless of the type of packaging, no contamination was revealed. This first study on the subject shows that, in presence of residual water, conservation of sterility is maintained for 14 days in the surgical set whether packaged in a container or nonwoven double packaging. These results provide the first answers to the possible use or non-use of humid compositions.
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Master Thesis
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Submitted on : Wednesday, October 15, 2014 - 3:49:28 PM
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  • HAL Id : dumas-01074834, version 1


Camille Fayard. Évaluation de l’impact de l’humidité résiduelle dans les plateaux opératoires après stérilisation. Sciences pharmaceutiques. 2014. ⟨dumas-01074834⟩



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