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Master Thesis

Mise en place et évaluation des dosages sériques de l’estradiol, de la progestérone et de l’hormone lutéinisante sur l’analyseur Dimension Vista (Siemens) : application aux dosages urinaires de l’estradiol et de l’hormone lutéinisante chez la femme au cours de l’aide médicale pour la procréation

Abstract : In order to enhance the organization of the biochemistry laboratory of Grenoble University Hospital Center we set new automated assay of estradiol, progesterone and luteinizing hormone (LH) in serum using the Dimension Vista 1500 which were previously analyzed by the Advia Centaur XP. The analytical performances of these new methods were compared to the reference values established by the French Society of Clinical Biology and data base of Carmen Ricos and col. Correlation between the two analyzers were evaluated. We wrote certification documents (SH FORM 43) for these assays according to the norm ISO NF EN 15189. Moreover, the impact of the new method on the turnaround time was evaluated. Besides, we use different way to evaluate the uncertainty of measurement. In addition, we studied the feasibility of urinary dosage of estradiol and LH to monitor women during medically assisted procreation. Imprecision (within and between run) is better with Vista analyzer. There are no difference between LH result. They are on average 20% lower for estradiol and 10% higher for progesterone. The new methods have improved the organization of the common platform of biochemistry. No changes in the turnaround time have been identified. The results of the urine tests showed that the urine is not a reliable matrix for the monitoring of patients in the context of medically assisted procreation.
Document type :
Master Thesis
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https://dumas.ccsd.cnrs.fr/dumas-01216528
Contributor : Jean-Hugues Morneau <>
Submitted on : Friday, October 16, 2015 - 2:05:55 PM
Last modification on : Tuesday, May 11, 2021 - 11:36:18 AM
Long-term archiving on: : Thursday, April 27, 2017 - 4:35:03 AM

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  • HAL Id : dumas-01216528, version 1

Citation

Hadrien Maguet. Mise en place et évaluation des dosages sériques de l’estradiol, de la progestérone et de l’hormone lutéinisante sur l’analyseur Dimension Vista (Siemens) : application aux dosages urinaires de l’estradiol et de l’hormone lutéinisante chez la femme au cours de l’aide médicale pour la procréation. Sciences pharmaceutiques. 2015. ⟨dumas-01216528⟩

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