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Master Thesis

Le diagnostic pré-implantatoire : comparaison de deux stratégies de biopsies embryonnaire, au stade clivé et au stade blastocyste

Abstract : PGT involves the genetic testing on cells from a biopsy of an embryo obtained by in vitro fertilization. There is a lack of standardization about the day chosen to perform the embryo biopsy country to country, even from center to center. In our center, biopsies are preferably performed on day 3 embryos cleaved but also at the blastocyst stage. The biopsy on day 5 requires almost systematically a freeze all before obtaining the diagnosis, unlike the biopsy on day 3 which allows an transfer of the healthy/balanced embryo from the next day or upon receipt of the genetic results. We wanted to compare the chances of success for couples supported in DPI in our center according to the day of the embryo biopsy. To achieve this, we carried out a retrospective monocentric study including all DPI cycles carried out between 2016 and 2019 divided into two groups, biopsies day 3 or 5. No significant difference emerges from the results concerning fresh embryo transfers. On the contrary, the rates of live birth and pregnancy are statistically higher when it comes to thawed embryo transfers. Regarding the efficiency of the two strategies, the rates of usefully and unnecessarily biopsied embryos were similar in the two groups of biopsies. In conclusion, even if our results in terms of births are favorable to biopsies on day 5, the generalization of this technique in our laboratory as done internationally seems hardly possible due to the many organizational and financial constraints that this would imply.
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https://dumas.ccsd.cnrs.fr/dumas-03019857
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Submitted on : Monday, November 23, 2020 - 3:40:38 PM
Last modification on : Thursday, January 21, 2021 - 3:10:31 AM
Long-term archiving on: : Wednesday, February 24, 2021 - 7:38:06 PM

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Julie Bacus. Le diagnostic pré-implantatoire : comparaison de deux stratégies de biopsies embryonnaire, au stade clivé et au stade blastocyste. Sciences du Vivant [q-bio]. 2020. ⟨dumas-03019857⟩

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