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Master Thesis

Validation d'une méthode de Multi-Target-Screening par chromatographie liquide couplée à la spectrométrie de masse en tandem et étude de l'utilisation des résultats des criblages toxicologiques dans les services de réanimation et d'urgences du CHU de Grenoble

Abstract : Toxicological screening is an essential analysis in hospital toxicology laboratories, as part of the management of severe drug poisoning in clinical situations where the history is unknown or with undefined diagnostic orientation. First, we validated a method of toxicological screening by high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) .The limits of identification was performed for 117 compounds. Variability in retention time and mass spectra were measured on a pool of 32 compounds. All coefficients of variation were analyzed below 20%. This toxicological screening was validated qualitatively but a semi-quantitative approach could not be validated. Then, we has evaluated prospectively over a period of 6 months, the use of toxicological screening results in intensive care units and emergency of University Hospital of Grenoble. Eighty-two screenings have been analyzed .Clinicobiological explanation has rationalized screenings requirements (23 % of prescriptions were canceled) . Ninety-seven percent of the toxicological screenings were considered useful and had an impact on patient care in 11.1 % of cases. Clinicobiological explanation is essential to help medical biologist to choose the right toxicological analysis strategy to implement. This discussion is the basis for a rapid, helpful and efficient toxicological analysis.
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Master Thesis
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https://dumas.ccsd.cnrs.fr/dumas-00910222
Contributor : Jean-Hugues Morneau <>
Submitted on : Wednesday, November 27, 2013 - 4:00:05 PM
Last modification on : Tuesday, May 11, 2021 - 11:36:16 AM
Long-term archiving on: : Monday, March 3, 2014 - 4:51:00 PM

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  • HAL Id : dumas-00910222, version 1

Citation

Gautier Szymanski. Validation d'une méthode de Multi-Target-Screening par chromatographie liquide couplée à la spectrométrie de masse en tandem et étude de l'utilisation des résultats des criblages toxicologiques dans les services de réanimation et d'urgences du CHU de Grenoble. Sciences pharmaceutiques. 2013. ⟨dumas-00910222⟩

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