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Master Thesis

L’ADN mitochondrial, nouvel acteur de l’immunité innée, est-il impliqué dans la polyarthrite rhumatoïde ? Détection, quantification et étude de sa source cellulaire

Abstract : Rheumatoid arthritis (RA) is an inflammatory and an auto-immune rheumatism, involving both innate and adaptive immunity. Recently, several studies underlined that mitochondrial DNA (mt DNA) was an activator of innate immunity and a local or systemic pro-inflammatory molecule. Indeed, in a mouse model, it was arthritogenic. Few studies focused on its involvement in RA. We analyzed plasmas and synovial fluids (SF) from 57 RA, by comparison with 11 crystal-induced arthritis (CA), 12 osteoarthritis (OA) and 15 healthy volunteers. SF from RA and CA contain significantly more mt DNA than OA SF (p=0,0007 et p<0,0001), as for plasmas from RA and CA in comparison with healthy plasmas (p=0,0237 et p=0,0302). In RA, synovial mt DNA rates increase with the disease clinical activity as measured by DAS 28-CRP, and correlate with CRP (r=0,82, p<0 ,0001), that has not been found for plasmatic rates. The number of polymorphonuclear neutrophils (PNN), main cell type of SF, correlates with the rate of SF mt DNA (r=0,75, p=0,0017). Moreover, intracellular mt DNA content in synovial PNN from RA is 4,59 lesser than the content from blood PNN, suggesting a local release of mt DNA from PNN. Mitochondrial DNA is then involved in inflammatory rheumatisms (RA and CA). It is a marker of inflammatory activity in joint, as for PNN, which likely represents the mt DNA cell source.
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Master Thesis
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https://dumas.ccsd.cnrs.fr/dumas-01096582
Contributor : Bu Carreire Université de Bordeaux <>
Submitted on : Wednesday, December 17, 2014 - 5:00:45 PM
Last modification on : Wednesday, August 23, 2017 - 4:41:25 PM
Long-term archiving on: : Monday, March 23, 2015 - 3:51:00 PM

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Anne Contis Chaleuil. L’ADN mitochondrial, nouvel acteur de l’immunité innée, est-il impliqué dans la polyarthrite rhumatoïde ? Détection, quantification et étude de sa source cellulaire. Médecine humaine et pathologie. 2014. ⟨dumas-01096582⟩

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