Nouvel outil d’analyse en cytométrie en flux : application à l’immunophénotypage de la LLC

Abstract : CLL is the most frequent leukemia in the world. Immunophenotyping by flux cytometry is fundamental for the diagnosis and follow-up of this disease : diagnosis uses the Matutes score and follow up uses MRD determination. However operator and biologist subjectivity remains inevitable during acquisition and analysis of flux cytometry data. This work is about finding a way to limit this subjectivity. It describes an analysis protocol elaborated with the help of the Flowsom software that aim to limit the inconveniences of conventional flux cytometry by proposing a different but complementary strategy to it. It has been established in the context of the immunophenotyping of a patient who had CLL. Results are encouraging, from cell populations numeration to the finding of the pathological population or even to the determination of positivity thresholds. The observation of cell populations is more complete and less subjective than in classical cytometry. This protocol is ready to be tested on a significant amount of samples in the context of CLL but also of non Hodgkinian B lymphomas. That will allow generation of important informations about the benefit of using this tool in medical biology. Depending on the results, antibody panels used in cytometry could be subject to evolution.
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Master Thesis
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Kavish Mohabeer. Nouvel outil d’analyse en cytométrie en flux : application à l’immunophénotypage de la LLC. Sciences pharmaceutiques. 2018. ⟨dumas-01931472⟩

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