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Master Thesis

Effets des néonicotinoides sur la neuroplasticité

Abstract : The extensive use of neonicotinoids, a class of widely used insecticides targeting the insect nicotinic receptors of the cholinergic system, raises concerns over its potential adverse effects in vertebrates. Indeed, the nicotinic receptors are widely distributed in the vertebrate peripheral and central nervous systems and play fundamental functions during development. In this study, we investigated the effects of a neonicotinoid: thiacloprid on central nervous system in parallel in zebrafish and mouse models. We exposed zebrafish larvae from 1 day post fertilization (dpf) to 6 dpf to 10-6 M, 10-7 M or 10-8 M thiacloprid. Whole heads were analyzed by RT-qPCR for endocrine (estrogen receptor alpha, estrogen receptor beta1 and beta2 and aromatase), proliferative (PCNA, neurogenin) and synaptic (synaptophysin, synapsin IIa and BDNF) markers. Then, we treated mice during gestation, from embryonic day 6 to 15 with 6mg/kg/day of thiacloprid via ingestion. 35 days old male and female offspring were euthanized and hippocampus, hypothalamus and amygdala were investigated for the expression of the same endocrine, proliferative and synaptic markers as those used in fish. We also analyzed the neuronal marker DCX (doublecortin). In the fish, the exposition to thiacloprid did not affect any of the markers. In mouse offspring, several markers were significantly affected in different brain areas.
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Submitted on : Friday, November 15, 2019 - 10:08:41 AM
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  • HAL Id : dumas-02364731, version 1



Valentine de l'Estoile. Effets des néonicotinoides sur la neuroplasticité. Sciences du Vivant [q-bio]. 2019. ⟨dumas-02364731⟩



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