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Master Thesis

Analyse du microbiome fongique et bactérien dans les sinusites fongiques chroniques

Abstract : In fungal ball rhinosinusitis, fungal hyphae are observed on direct examination (DE) of biopsy samples but culture remains negative in most cases. This could be the result of microbial interactions weakening the fungus. The aim of this study was to describe and analyze bacterial and fungal microbiota by targeted metagenomics in fungal balls and find possible microbial. A total of 44 sinus biopsies were studied from 44 patients who underwent surgery for chronic rhinosinusitis from 2015 to 2017 in Paris. DNA was extracted and fungal ITS2 loci and bacterial V3-V4 16S ribosomal DNA locus were amplified and sequenced (MiSeqTM Illumina). Microbial diversity was analyzed with QIIME, associated with UNITE and SILVA databases for ITS and 16S, respectively. These data were compared to microbiological and clinical data. Among these 44 patients, 37 had fungal balls and 7 had non-fungal chronic rhinosinusitis (controls). Among the 37 fungal balls, DE and culture were positive for fungi in 97.2% and 32.4% of biopsies, respectively. Targeted metagenomic analysis yielded 56 fungal taxa and 122 bacterial taxa. Using principal coordinate analysis (Bray-Curtis distance; permanova test), a significantly different bacterial and fungal metagenomic profiles in patients with fungal balls compare to controls was found (p=0.047 and p<0.001, respectively). Aspergillus was predominantly identified in 89.2% of fungal balls. One bacterial taxon, Hæmophilus, was significantly over-represented in patients with fungal balls compared to controls (p<0.001). Fungal balls pathogenesis remains unclear and could result from microbial interactions between fungi and bacteria in mixed biofilm-like structure.
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Master Thesis
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https://dumas.ccsd.cnrs.fr/dumas-03143885
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Submitted on : Wednesday, February 17, 2021 - 10:09:11 AM
Last modification on : Thursday, September 2, 2021 - 3:09:13 AM

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  • HAL Id : dumas-03143885, version 1

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Sarah Dellière. Analyse du microbiome fongique et bactérien dans les sinusites fongiques chroniques. Médecine humaine et pathologie. 2019. ⟨dumas-03143885⟩

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