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Master Thesis

Les cellules souches issues de la gelée de Wharton, culture dynamique et contrôle qualité

Abstract : The therapeutic potential of mesenchymal stem cells (MSC) raises an increasing interest in regenerative medicine. For such needs, Wharton’s jelly stem cells are an alternative source to bone marrow stem cells, by their collection, their abundance and their internal features. Thanks to their self-renewal and differentiation properties, expansion, and thus obtaining a sufficient number of cells, allows numerous therapeutic applications. However, mesenchymal stromal cells expansion faces today a number of scientific and technological challenges to ensure their use on a massive scale in cell therapy or tissue engineering. To this end, the implementation of robust culture processes that jointly guarantee increased expansion performance and consistent cell quality is necessary. For such a purpose, the use of adhesion supports (microcarriers) suspended in a bioreactor is therefore now favored. Regardless of the expansion method, each production step can potentially alter cell quality. The identification of critical quality attributes, and the implementation of tests to control them is essential for the release of production batches, in order to ensure the safety and effectiveness of the final product.
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Master Thesis
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https://dumas.ccsd.cnrs.fr/dumas-03160848
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Submitted on : Friday, March 5, 2021 - 3:31:30 PM
Last modification on : Thursday, April 15, 2021 - 3:11:41 AM
Long-term archiving on: : Sunday, June 6, 2021 - 7:05:20 PM

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  • HAL Id : dumas-03160848, version 1

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Anaïs Laborde. Les cellules souches issues de la gelée de Wharton, culture dynamique et contrôle qualité. Sciences du Vivant [q-bio]. 2021. ⟨dumas-03160848⟩

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