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Master Thesis

Étude de la mise en œuvre du test d'activation des basophiles pour le diagnostic des hypersensibilités médicamenteuses

Abstract : The basophil activation test (BAT) is complementary to skin tests (ST) and specific IgE assay (sIgE) and an alternative to the provocation test. This ex vivo test determines the activation of basophils by flow cytometry. The objective of this work was to assess the implementation and diagnostic contribution of the TAB for drug hypersensitivity at University Hospital of Bordeaux. Secondly, the objective was to assess current practices and procedures of BAT in French laboratories. We retrospectively analyzed the records of patients for whom TABs for drugs were requested between 2015 and 2020 at University Hospital of Bordeaux. To evaluate the current practices, we conducted a national survey (AllergoBioNet network). Information’s requested were : annual prescription volume, procedures, indications and interpretation. We observed a diagnostic contribution in 50% of the records analyzed. In Bordeaux, the total number of BAT is increasing since 2017. They mainly concern antibiotics (57%) and curares (19%). The 2 major indications were a discrepancy between the clinical history and the result of ST and/or sIgE (27%) or inconclusive or unavailable ST and/or sIgE (28%). The French laboratories perform BAT from whole blood (EDTA) (92.9%), sent in less than 24h (100%) using a commercial kit (100%). The positivity thresholds and BAT interpretation remained very variable. Developing the BAT for the diagnosis of drug allergy makes sense because of its diagnostic contribution. At the national level, the standardization of BAT interpretation is necessary.
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Master Thesis
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https://dumas.ccsd.cnrs.fr/dumas-03188773
Contributor : Frédérique Gambade <>
Submitted on : Friday, April 2, 2021 - 2:19:18 PM
Last modification on : Thursday, May 13, 2021 - 3:18:15 AM

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Juliette Charpy. Étude de la mise en œuvre du test d'activation des basophiles pour le diagnostic des hypersensibilités médicamenteuses. Sciences du Vivant [q-bio]. 2020. ⟨dumas-03188773⟩

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