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Master Thesis

Retrospective analysis of multiplex PCR results and their impact in current practice

Abstract : Background: hospital-acquired pneumonia (HAP) and ventilator-associated pneumonia (VAP) are among leading causes of in-hospital infectious complications. They are associated with a significant rise in morbidity and intensive care unit (ICU) length of stay. Early and adequate antibiotherapy is a cornerstone of their management. Empirical broad-spectrum antibiotherapy is mandatory until definitive bacterial identification and antibiotic susceptibility testing results. During the last years, laboratory bacterial diagnosis saw the emergence of rapid multiplexed-Polymerase Chain Reaction (rm-PCR) syndromic molecular diagnosis assays for pneumonia giving results with much shorter turnaround times compared to actual standard bacterial diagnosis methods. This innovative rm-PCR diagnosis technique could yield interesting bacteriologie results that could help clinicians to earlier introduce effective antibiotics and to reduce wide-spectrum antibiotics use by quick antibacterial spectrum adaptation. Information upon clinical performance and routine use of this novative method is stilllacking. The main objective of this study was to evaluate the clinical performance of this panel versus standard bacteriological methods in a clinical setting.
Methods: we retrospectively examined medical data of 103 VAP and HAP suspicions occurring between March 20, 2020 and April30, 2021 in ICU-patients from 3 different teaching hospitals. We compared results from conventional bacteriology and from rm-PCR while examining the clinical evolution and treatment strategy modifications in response to bacteriologie results to analyze impact on antibiotherapy.
Results : data from 103 pneumonia episodes (48 patients) were analyzed.Conclusions : Rm-PÇR provides ICU clinicians with mostly safe and useful results in significantly quicker turnaround times. More clinical evidence is needed ta gain trust in this technique.
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Master Thesis
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Contributor : Bibliothèque Universitaire de Médecine Nice Connect in order to contact the contributor
Submitted on : Wednesday, January 5, 2022 - 2:42:30 PM
Last modification on : Tuesday, April 12, 2022 - 3:46:44 AM


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  • HAL Id : dumas-03512646, version 1


Léopold Letouzé. Retrospective analysis of multiplex PCR results and their impact in current practice. Human health and pathology. 2021. ⟨dumas-03512646⟩



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