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Master Thesis

Aspergilloses invasives et Mucormycoses : revue bibliographique et évaluation diagnostique du kit de PCR Aspergillus spp.-Mucorales Mycogénie®

Abstract : Mucormycosis and invasive aspergillosis are serious infections which are most often associated with nonspecific symptoms and therefore requires an early and appropriate management. Improving diagnostic methods is a key challenge for therapy optimization and reducing mortality rates associated with these diseases. This thesis is divided into two parts. First, a literature review describes the causative agents of these infections, their physiopathology and the current microbiological diagnostic tools, including the molecular biology techniques recently included in the 2019 EORTC / MSGERC recommendations for Aspergillus. Furthermore, we assessed the analytical performance and diagnostic value of the recently marketed Aspergillus spp.-Mucorales Mycogénie® PCR kit. By comparing the results obtained from 101 samples selected retrospectively and prospectively using this kit with other diagnostic methods (mycological culture, reference PCR, galactomannan antigens for Aspergillus), we obtained satisfactory results that allowed the implementation of this new technique at the Mycology Laboratory of the Bordeaux Hospital University Center. This study highlights the benefits of combining several samples and diagnostic methods as well as comparing the results to the clinical context in order to provide the most accurate interpretation possible. Therefore, these molecular biology techniques must be used to balance the strengths and limitations of other techniques in the diagnosis of invasive aspergillosis and mucormycosis.
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Master Thesis
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https://dumas.ccsd.cnrs.fr/dumas-03557660
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Submitted on : Friday, February 4, 2022 - 2:40:40 PM
Last modification on : Saturday, February 5, 2022 - 3:38:54 AM
Long-term archiving on: : Thursday, May 5, 2022 - 7:03:20 PM

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Pharmacie_2021_Portejoie.pdf
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Laure Portejoie. Aspergilloses invasives et Mucormycoses : revue bibliographique et évaluation diagnostique du kit de PCR Aspergillus spp.-Mucorales Mycogénie®. Sciences du Vivant [q-bio]. 2021. ⟨dumas-03557660⟩

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