HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Master Thesis

Développement d'un bioréacteur pour l'étude ex vivo de biomatériaux appliqués à l'odontologie : élaboration d’un cahier des charges à partir d’une revue systématique de la littérature

Abstract : Introduction: to our knowledge, there is currently no efficient ex vivo model for the study of the healing mechanisms of the dentin-pulp complex and the properties of pulp capping materials. The objective of this work was to list the different ex vivo models used for the study of materials aiming at pulp repair and regeneration, in order to establish specifications for the development of a new efficient ex vivo study model. Material and method: a systematic review of the literature was performed using two databases (Pubmed® and Scopus®). Studies had to concern the ex vivo study of the properties of biomaterials or tissue engineering strategies for conservative dentistry using extracted human teeth. Results: 19 articles were included in this study. During the analysis, several parameters were identified: the types of models used, the methods of model preparation, the methods of model culture and the means of evaluating cellular and/or tissue behavior. Discussion: different models are found in the literature. Although they aim to approximate physiological conditions, they have certain limitations. The diffusion of the culture medium to the cells of the dental pulp being limited, cells have a restricted access to the elements necessary for their development. Therefore, the development of a new model based on the design of a bioreactor seems relevant. The review of the literature has allowed us to identify the paths to follow for the design of an optimal model. Another study on the analysis of the viability and the cellular behavior after periods of culture higher than 4 weeks within this bioreactor is necessary. This will validate the relevance of this bioreactor model for the study of ex vivo pulp capping biomaterials.
Complete list of metadata

https://dumas.ccsd.cnrs.fr/dumas-03586187
Contributor : Odonto Ub - Connect in order to contact the contributor
Submitted on : Wednesday, February 23, 2022 - 4:34:00 PM
Last modification on : Tuesday, March 1, 2022 - 3:30:14 AM

File

Odonto_2022_Maisani.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : dumas-03586187, version 1

Collections

Citation

Mathieu Maisani. Développement d'un bioréacteur pour l'étude ex vivo de biomatériaux appliqués à l'odontologie : élaboration d’un cahier des charges à partir d’une revue systématique de la littérature. Sciences du Vivant [q-bio]. 2022. ⟨dumas-03586187⟩

Share

Metrics

Record views

20

Files downloads

5