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Master Thesis

Impact de faibles doses de cannabidiol sur la douleur neuropathique et les symptômes de type anxio-dépressifs chez la souris

Abstract : Neuropathic pain is defined as a long-lasting pain caused by an injury or a disease of the somatosensory system. It is associated with numerous comorbidities such as anxiety and depressive disorders. First-line treatments fail in 15-20 % of patients due to adverse effects, so the search for alternative treatments is essential. Cannabidiol (CBD) has analgesic, anxiolytic and antidepressant properties, but the mechanisms of action are not fully elucidated. In this context, this veterinary thesis is part of Marion Gaborit's university thesis, a project with two objectives. The first goal was to determine whether the δ-opioid receptor is required to observe these properties in mice suffering from neuropathic pain (sciatic nerve compression model) using genetically modified male and female mice expressing this receptor or not. Behavioral tests were performed to measure mechanical allodynia and anxio-depressive-like behaviors. The second goal was to map by immunohistochemistry the neuronal co-localization of the δ-opioid and CB1-cannabinoid receptors in neuropathic mouse brains. This will provide a better understanding of their functional interactions in the neuropathic context. Our preliminary results in males suggest that the analgesic effect of CBD requires the δ-opioid receptor because the effect was not observable in its absence. For now, we cannot conclude whether CBD has an effect on anxio-depressive-like behaviors or not. Finally, we observed co-localized δ and CB1 receptors in the rostral ventromedial medulla (RVM), a structure involved in the descending modulation of pain, and in the dorsal raphe nucleus, involved in mood regulation.
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Master Thesis
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https://dumas.ccsd.cnrs.fr/dumas-03633905
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Submitted on : Thursday, April 7, 2022 - 12:14:18 PM
Last modification on : Thursday, April 21, 2022 - 3:57:52 PM

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Coline Sagot. Impact de faibles doses de cannabidiol sur la douleur neuropathique et les symptômes de type anxio-dépressifs chez la souris. Sciences du Vivant [q-bio]. 2022. ⟨dumas-03633905⟩

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