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Master Thesis

Utilisation des cellules souches mésenchymateuses humaines pour la thérapie cellulaire anti-cancéreuse dans un modèle de glioblastome

Abstract : The difficulty in cancer treatment, especially gliomas, is linked to the low level of therapeutic molecules brought to the tumor site. Current treatments for glioblastomas, the most common adult malignant tumor, result in a median survival of only 14 months. The prognosis of patients with malignant gliomas, such as glioblastoma multiforme, is extremely poor, despite their extensive surgical treatment and adjuvant radio and chemo therapy. The ability of bone marrow-derived stem cells to migrate to areas of injury in a range of pathologic conditions suggests that they may be ideal vectors for therapeutic delivery. In our study, we evaluated the power of human Mesenchymal Stem Cells (hMSC) themselves as a diagnostic and a therapeutic tool in cancer therapy. Using highly malignant and invasive human glioma models, we followed, in vivo, by bioluminescence optical imaging and by magnetic resonance imaging (MRI), hMSC migration on tumor bearing mice after systemic injection. Nude mice were engrafted with human glioblastoma cells and hMSC cells were systemically injected. The tumor progression was evaluated using MRI or optical bioluminescence imaging. Our study demonstrated that hMSC injected systemically have a tropism for tumor site, migrate extensively to the tumor and remain alive inside the tumor. Moreover, the tumor growth is slown down only thanks to the presence of hMSC themselves and this phenomenon seems to be dose-dependent. This study, performed in conditions as close as possible from the human clinic, reveals a brilliant future for the glioblastoma treatment.
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Master Thesis
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https://dumas.ccsd.cnrs.fr/dumas-00593130
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Submitted on : Friday, May 13, 2011 - 1:55:05 PM
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  • HAL Id : dumas-00593130, version 1

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Anastassia Karageorgis. Utilisation des cellules souches mésenchymateuses humaines pour la thérapie cellulaire anti-cancéreuse dans un modèle de glioblastome. Sciences pharmaceutiques. 2010. ⟨dumas-00593130⟩

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