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Master Thesis

Caractérisation du dialogue entre Streptococcus thermophilus et le tractus gastro-intestinal de son hôte

Abstract : My internship in the ProbiHôte laboratory of the Micalis unit has focused on the characterization of the dialogue between the bacteria Streptococcus thermophilus and the gastrointestinal tract (GIT). To study this dialogue, I worked on a gnotobiotic animal model carrying only one bacteria in its GIT. I have investigated the main effects undergone by the GIT in response to bacterial colonization and the adaptation process developed by S. thermophilus in the course of its implantation. I demonstrated a boost of disaccharide metabolism in S. thermophilus after implantation. Knowing that as a first step of low population occurs the first week after inoculation of the animals, I have suggested an adaptation process during this time which selects bacteria based on their metabolic properties. Drawing from my laboratory's previous results, I have confirmed an enhancing effect of S. thermophilus on mucus cell differentiation, and I have suggested a role of the TNFa and TGFß signaling cell factors also involved in the anti-inflammatory response. Likewise, we have checked our model on conventional flora colonized animals in which a deficiency in mucus production induces colitis. For the purpose of following bacterial implantation among the microbiota, I have constructed a kanamycin-resistant mutant of S. thermophilus.
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Master Thesis
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Submitted on : Thursday, March 7, 2013 - 11:16:02 AM
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Félix Portello. Caractérisation du dialogue entre Streptococcus thermophilus et le tractus gastro-intestinal de son hôte. Sciences agricoles. 2012. ⟨dumas-00797791⟩

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