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Master Thesis

Multiplication des mycorhizes arbusculaires en milieu liquide et solide afin d'améliorer la formulation de biofertilisants

Abstract : With the increased use of chemical fertilizers in Latin America and the side effects trained, microbiology research develops. Thus, PROINPA foundation headquartered in Cochabamba (Bolivia), is producing organic fertilizers, based on arbuscular mycorrhiza (AM). The objective of this work is to increase the AM minimizing the amount of substrate used. Two techniques are tested here, the multiplication of AM substrate base of clay and / or sand and hydroponics culture. The host, hordeum vulgare, is stressed by the shallow depth of the substrate or by the lack of nutrients in the solution to cause sporulation: S100, S50 and T, respectively 100%, 50% and 0% of nutrient solution are tested. Evaluated variables are agronomic data such germination, plant height and root dry weight, and data on the successful establishment of symbiosis or not, with the study of colonization frequency and spores number identified by sample. Results in solid medium gave negative rates of multiplication, which means that the inoculated spores germinated but the experimental conditions were not suitable for the establishment of symbiosis. The sand could give better results in terms of root development and thus colonization but by testing greater depths. In contrast, the rate of increase in hydroponics are very satisfactory (up to 1271%), especially in S100. It remains to improve the protocol formulation AM and study the adaptation of the product under actual conditions of culture.
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Master Thesis
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Submitted on : Monday, April 7, 2014 - 5:08:59 PM
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  • HAL Id : dumas-00975007, version 1



Laure Priou. Multiplication des mycorhizes arbusculaires en milieu liquide et solide afin d'améliorer la formulation de biofertilisants. Sciences agricoles. 2013. ⟨dumas-00975007⟩



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