Local early response to an MVA-mediated vaccination: effects of an MVA infection on skin fibroblasts and macrophages

Abstract : MVA is an attenuated poxvirus frequently used in vaccine strategies. Previous results from the lab show unknown dermis cells expressing viral proteins. Those unidentified dermis cells have a morphology alike fibroblasts and/or macrophages. As MVA is routinely used as a recombinant vector in Non-human primates, this model is used here to identify dermis fibroblasts and monocyte-derived macrophages as infection targets of MVA, their cellular response to MVA and their immune role in the response to an MVA infection. Fibroblasts are cultured from skin biopsies and macrophages are derived from peripheral blood monocytes, then cell cultures are incubated with MVA-eGFP. Cells’ activity changes are monitored by flow cytometry and Multiplex® immunoassay on medium samples. The results seem to indicate an effective infection of fibroblasts, higher with culture time after infection, along with MVA-induced cell death. Macrophages do not appear infected but their activations markers undergo some changes in their expression. Both cell types, either infected or not, appear to show different cytokine secretions in presence of MVA.
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Master Thesis
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Submitted on : Wednesday, July 15, 2015 - 11:54:13 AM
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Julie Jouffrey. Local early response to an MVA-mediated vaccination: effects of an MVA infection on skin fibroblasts and macrophages. Life Sciences [q-bio]. 2015. ⟨dumas-01176350⟩

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