Skip to Main content Skip to Navigation
Master Thesis

Problématique diagnostique d'infection par un virus dengue en zone endémo-épidémique (Guadeloupe) et élaboration de contrôles internes de la qualité pour les tests AgNS1 et IgM

Abstract : Dengue virus, the most widespread arbovirosis on earth, circulates in the Caribbean and in particular in Guadeloupe in an endemo-epidemic mode. Vectors for disease are Aedes mosquitoes among which, Aedes aegypti and Aedes albopictus are the two most important. Clinical signs of dengue virus infection are often nonspecific and their clinical evolution is unpredictable. Usually mild, serious or even fatal forms may occur. Currently, the three diagnostic methods used are the detection of the viral genome using nucleic acids amplification technology (RT-PCR), detection of dengue virus specific antigens NS1 and indirect serological diagnosis. Using them together or individually depends on the clinical stage of the disease, the date of onset of clinical signs and the epidemiological context. There is no specific antiviral treatment or vaccine available at this time. Treatment is symptomatic. Issuing reliable biological results for the dengue diagnosis requires implementing internal quality control (IQC). For that purpose, in the laboratory of virology of the teaching hospital of Pointe-à-Pitre, we have developed an in house CIQ for the NS1 antigen detection and a CIQ for dengue IgM serological detection from human sera. Three matrices have been used for dilutions : Phosphate-Buffered Saline, Fetal Bovine Serum and human serum. This study showed that the Fetal Bovine Serum presented more reliable results in terms of repeatability (Coefficient of variation CV 14 % for the AgNS1 and 4 % for the IgM anti-dengue) and of reproducibility (CV 20,8 % for the AgNS1 and 13,8 % for the IgM anti-dengue). Even if they seem rather high, these values of CV have to be linked with the type of techniques used and expectations of reproductibility (semi-quantitative methods for both studied parameters).
Document type :
Master Thesis
Complete list of metadata

https://dumas.ccsd.cnrs.fr/dumas-01191535
Contributor : Bu Carreire Université de Bordeaux <>
Submitted on : Wednesday, September 2, 2015 - 9:54:44 AM
Last modification on : Wednesday, August 23, 2017 - 4:30:51 PM
Long-term archiving on: : Wednesday, April 26, 2017 - 7:03:36 PM

Identifiers

  • HAL Id : dumas-01191535, version 1

Collections

Citation

Clarisse Benard. Problématique diagnostique d'infection par un virus dengue en zone endémo-épidémique (Guadeloupe) et élaboration de contrôles internes de la qualité pour les tests AgNS1 et IgM. Sciences pharmaceutiques. 2015. ⟨dumas-01191535⟩

Share

Metrics

Record views

178

Files downloads

1784