Skip to Main content Skip to Navigation
Master Thesis

Découverte de nouveaux chalcophores de type méthanobactine chez les non-méthanotrophes : construction d’un système d’expression hétérologue

Abstract : Transition metals such as copper and iron are essentials for bacterial survival in their ecological niches. The methanobactins produced by the methanotroph bacteria are the only example of chalcophores that specifically chelate copper with high affinity. They play an important role in the physiology and the ecology of the methanotrophs which have a strong need for copper in order to realize the oxidation of methane. Genome analyses revealed that the related chalcophores exist in some non-methanotroph bacteria such as Pseudomonas extrmaustralis. In order to understand the chemo-diversity of these peptides, this internship aimed to develop a system of heterologous expression in Escherichia coli for the gene cluster of the methanobactine-like peptide originating from P. extremaustralis. We have established a cloning protocol based on the homologous recombination in Sacharomyces cerevisiae and have applied this method to insert the whole gene cluster into an expression vector. The obtained results open the perspective to discover and characterize the related methanobactin-like peptide.
Document type :
Master Thesis
Complete list of metadatas

Cited literature [147 references]  Display  Hide  Download

https://dumas.ccsd.cnrs.fr/dumas-01452284
Contributor : Bu Carreire Université de Bordeaux <>
Submitted on : Wednesday, February 1, 2017 - 5:34:19 PM
Last modification on : Wednesday, August 23, 2017 - 4:29:56 PM

Identifiers

  • HAL Id : dumas-01452284, version 1

Collections

Citation

Victor Sayous. Découverte de nouveaux chalcophores de type méthanobactine chez les non-méthanotrophes : construction d’un système d’expression hétérologue. Sciences pharmaceutiques. 2016. ⟨dumas-01452284⟩

Share

Metrics

Record views

113

Files downloads

86