Recherche par génétique d'association de gènes impliqués dans l'interaction pois x rhizobium

Abstract : Nitrogen is an important resource in agronomy; its massive use in the current agronomy has a negative impact on the environment. Legumes have the dual benefit of allowing the production of high-protein seeds without the need for nitrogen fertilizer. The cultivation of legumes, including pea, the most cultivated species in France, however, remains limited, mainly because of the irregularity of their yield. The challenge of the SYMBIOPEA project is to improve the symbiosis between pea and its symbiotic nitrogen-fixing partner Rhizobium leguminosarum sv. viciae (Rlv). This project is based on association genetic analyzes and aims to find genes or QTLs with an impact on nodulation and interaction between pea and rhizobium through a study of a diverse panel of pea accessions inoculated with a mixture of Rlv strains. For each of the variables studied, different mixed models each based on a different type of structuring were tested. All the structures tested were consistent. The choices for each of the Rlv strains are variables that are difficult to analyze by association genetics because they have a rather qualitative profile. As a result, a profile of choice among the Rlv strains was established from a principal component analysis. SNPs in linkage disequilibrium with putative genes of interest were identified for plant growth, the pivot length bearing nodules, and strain selection profile variable.
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Master Thesis
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Valentin Delefortrie. Recherche par génétique d'association de gènes impliqués dans l'interaction pois x rhizobium. Sciences du Vivant [q-bio]. 2018. ⟨dumas-02080506⟩

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