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Master Thesis

Évaluation des performances du test de quantification de l'ADN du virus de l'hépatite B par GeneXpert Cepheid : application dans la qualification microbiologique des dons d'organes en urgence

Abstract : According to the World Health Organization, 30% of the world’s population has been infected with hepatitis B virus (HBV), responsible for nearly 700,000 deaths a year. Eligibility criteria for organ donation require HBV testing, like HIV and HCV, which represent an average of 160 screenings per year in Bordeaux Hospital. Accredited laboratories have to provide the results of viral genome screenings, as quickly as possible before transplantation. Recently, new “point-of-care” molecular tests based on single-use cartridges have been developed, allowing rapid viral load (VL) quantification. The aim of our study was to assess the performance of a new point-of-care molecular test for HBV DNA quantification, GeneXpert® Cepheid. The linearity of this quantitative assay was confirmed using serial dilutions of different HBV genotypes samples (r = 0.951 to 1.035). The Xpert HBV results were positively correlated with the reference method Roche HBV Cobas 6800 (r=0.993) for viral loads ranging from 1.46 to 9 log10IU/mL. Among the 93 tested samples, 88 samples (94.6%) showed good agreements between the 2 methods. Three HBV VLs estimated< 10 IU/mL by Cobas 6800 Roche were not detected by HBV Xpert. In conclusion, this test provides HBV viral load results in less than one hour, demonstrates excellent performances and is easy to use, even by unqualified operators, making it suitable for emergency situations. Since its implementation in Bordeaux Hospital, the time to provide HBV VL results for assessment of donor eligibility has been reduced by 20 fold (2.69 hours on average versus 55 hours previously).
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Master Thesis
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https://dumas.ccsd.cnrs.fr/dumas-02337418
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Submitted on : Tuesday, October 29, 2019 - 2:21:15 PM
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Jessica Cros-Labrit. Évaluation des performances du test de quantification de l'ADN du virus de l'hépatite B par GeneXpert Cepheid : application dans la qualification microbiologique des dons d'organes en urgence. Sciences du Vivant [q-bio]. 2019. ⟨dumas-02337418⟩

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